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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse thymus tissue using OCT2-Specific Polyclonal antibody (18996-1-AP)

    WB analysis of mouse thymus using 18996-1-AP (same clone as 18996-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18996-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using OCT2-Specific Polyclonal antibody (18996-1-AP)

    WB analysis of mouse kidney using 18996-1-AP (same clone as 18996-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18996-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using OCT2-Specific Polyclonal antibody (18996-1-AP)

    WB analysis of Raji using 18996-1-AP (same clone as 18996-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18996-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using OCT2-Specific Polyclonal antibody (18996-1-AP)

    WB analysis of human brain using 18996-1-AP (same clone as 18996-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18996-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human spleen tissue using OCT2-Specific Polyclonal antibody (18996-1-AP)

    WB analysis of human spleen using 18996-1-AP (same clone as 18996-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18996-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using OCT2-Specific Polyclonal antibody (18996-1-AP)

    IHC staining of human spleen using 18996-1-AP (same clone as 18996-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 18996-1-AP (OCT2-Specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18996-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using OCT2-Specific Polyclonal antibody (18996-1-AP)

    IHC staining of human spleen using 18996-1-AP (same clone as 18996-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 18996-1-AP (OCT2-Specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18996-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

OCT2-Specific Polyclonal antibody, PBS Only

OCT2-Specific Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 18996-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

OCT2, POU2F2, Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2, Oct-2, Octamer-binding protein 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18996-1-PBS targets OCT2-Specific in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name POU class 2 homeobox 2
Calculated molecular weight 62 kDa
Observed molecular weight52-66 kDa
GenBank accession numberNM_002698
Gene Symbol POU2F2
Gene ID (NCBI) 5452
RRIDAB_10596624
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP09086
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

OCT2, also named as POU2F2 and OTF2, belongs to the POU transcription factor family and Class-2 subfamily. OCT2 is a transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). OCT2 regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. It modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter. The antibody is specific to OCT2. OCT2 can be detected 52-66 kDa (PMID: 15377492, PMID: 34010659).

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