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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OCIAD2 Polyclonal antibody (13437-1-AP)

    WB analysis using 13437-1-AP

    U-251 cells were subjected to SDS PAGE followed by western blot with 13437-1-AP (OCIAD2 antibody) at dilution of 1:1000 and incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using OCIAD2 Polyclonal antibody (13437-1-AP)

    IHC staining of human stomach cancer using 13437-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13437-1-AP (OCIAD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using OCIAD2 Polyclonal antibody (13437-1-AP)

    IHC staining of human stomach cancer using 13437-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13437-1-AP (OCIAD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using OCIAD2 Polyclonal antibody (13437-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 13437-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 13437-1-AP (OCIAD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using OCIAD2 Polyclonal antibody (13437-1-AP)

    IF Staining of U-251 using 13437-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using OCIAD2 antibody (13437-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

OCIAD2 Polyclonal antibody

OCIAD2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13437-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Ovarian carcinoma immunoreactive antigen-like protein, OCIA domain-containing protein 2, OCIA domain containing 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU-251 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13437-1-AP targets OCIAD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4243

Product name: Recombinant human OCIAD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-99 aa of BC032808

Sequence: MASASARGNQDKDAHFPPPSKQSLLFCPKSKLHIHRAEISKIMRECQEESFWKRALPFSLVSMLVTQGLVYQGYLAANSRFGSLPKVARTASLPVRNAK

相同性解析による交差性が予測される生物種
Full Name OCIA domain containing 2
Calculated molecular weight 99 aa, 11 kDa
Observed molecular weight17 kDa
GenBank accession numberBC032808
Gene Symbol OCIAD2
Gene ID (NCBI) 132299
RRIDAB_3085414
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ56VL3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for OCIAD2 antibody 13437-1-APDownload protocol
IHC protocol for OCIAD2 antibody 13437-1-APDownload protocol
IF protocol for OCIAD2 antibody 13437-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols