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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OCIAD1 Polyclonal antibody (16634-1-AP)

    WB analysis using 16634-1-AP (same clone as 16634-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16634-1-AP (OCIAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16634-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using OCIAD1 Polyclonal antibody (16634-1-AP)

    IP experiment of HeLa using 16634-1-AP (same clone as 16634-1-PBS)

    IP result of anti-OCIAD1 (IP:16634-1-AP, 4ug; Detection:16634-1-AP 1:300) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 16634-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using OCIAD1 Polyclonal antibody (16634-1-AP)

    IHC staining of human liver cancer using 16634-1-AP (same clone as 16634-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16634-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using OCIAD1 Polyclonal antibody (16634-1-AP)

    IHC staining of human liver cancer using 16634-1-AP (same clone as 16634-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16634-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using OCIAD1 Polyclonal antibody (16634-1-AP)

    IHC staining of human thyroid cancer using 16634-1-AP (same clone as 16634-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16634-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using OCIAD1 Polyclonal antibody (16634-1-AP)

    IHC staining of human thyroid cancer using 16634-1-AP (same clone as 16634-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16634-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using OCIAD1 Polyclonal antibody (16634-1-AP)

    IHC staining of human kidney using 16634-1-AP (same clone as 16634-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16634-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using OCIAD1 Polyclonal antibody (16634-1-AP)

    IHC staining of human kidney using 16634-1-AP (same clone as 16634-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16634-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using OCIAD1 Polyclonal antibody (16634-1-AP)

    IHC staining of human pancreas cancer using 16634-1-AP (same clone as 16634-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16634-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using OCIAD1 Polyclonal antibody (16634-1-AP)

    IHC staining of human pancreas cancer using 16634-1-AP (same clone as 16634-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16634-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using OCIAD1 Polyclonal antibody (16634-1-AP)

    IF Staining of HeLa using 16634-1-AP (same clone as 16634-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OCIAD1 antibody (16634-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 16634-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using OCIAD1 Polyclonal antibody (16634-1-AP)

    IF Staining of HepG2 using 16634-1-AP (same clone as 16634-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using OCIAD1 antibody (16634-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 16634-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16634-1-PBS targets OCIAD1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OCIAD1 fusion protein Ag9977 相同性解析による交差性が予測される生物種
Full Name OCIA domain containing 1
Calculated molecular weight 28 kDa
Observed molecular weight 29-35 kDa
GenBank accession numberBC003409
Gene Symbol OCIAD1
Gene ID (NCBI) 54940
RRIDAB_2156297
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NX40
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

OCIAD1 was first identified by immunoscreening of an ovarian carcinoma cDNA expression library with ascites fluid from ovarian cancer patients (PMID: 11162530). OCIAD1 has been reported as a key player in ovarian cancer cell adhesion, as well as a key player in generating ovarian cancer recurrence (PMID: 18328549; 20515946). In addition to its roles in cancer, OCIAD1 participates in maintaining stem cell potency by regulating the Jak/STAT pathway (PMID: 23972987). Several alternatively spliced forms of OCIAD1 gene have been identified. The longest form (1.4 kb) is predicted to encode for a 27.6 kDa protein of 245 amino acids. This antibody detects OCIAD1 with an apparent molecular weight of ~35 kDa as has been demonstrated by several researches (PMID: 27345969; 27345976).