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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OAT Polyclonal antibody

OAT Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17089-1-AP

Synonyms

HOGA, OAT

Validation Data Gallery

Applications:
  • IHC staining of human liver cancer using 17089-1-AP

    IHC staining of human liver cancer using 17089-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17089-1-AP (OAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 17089-1-AP

    IHC staining of human liver cancer using 17089-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17089-1-AP (OAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 17089-1-AP

    IF Staining of HepG2 using 17089-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 17089-1-AP (OAT antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17089-1-AP targets OAT in IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OAT fusion protein Ag9981 相同性解析による交差性が予測される生物種
Full Name ornithine aminotransferase (gyrate atrophy)
Calculated molecular weight 62 kDa
GenBank accession numberBC000964
Gene symbol OAT
Gene ID (NCBI) 4942
RRIDAB_2878347
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for OAT antibody 17089-1-APDownload protocol
IF protocol for OAT antibody 17089-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols