Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, PC-3 cells, HSC-T6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27009-1-AP targets Nurim in WB, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25501 Product name: Recombinant human NRM protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-98 aa of BC039865 Sequence: MAPALLLIPAALASFILAFGTGVEFVRFTSLRPLLGGIPESGGPDARQGWLAALQDRSILAPLAWDLGLLLLFVGQHSLMAAERVKAWTSRYFGVLQR 相同性解析による交差性が予測される生物種 |
| Full Name | nurim (nuclear envelope membrane protein) |
| Calculated molecular weight | 29 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC039865 |
| Gene Symbol | NRM |
| Gene ID (NCBI) | 11270 |
| RRID | AB_3742209 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IXM6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Nurim (nuclear envelope membrane protein) is also known as NRM and NRM29. Its primary cellular localization is the inner nuclear membrane (INM), where it is tightly associated with the nucleus. Nurim has emerged as a crucial regulator of apoptosis suppression.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Nurim antibody 27009-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |