Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
26983-1-AP targets Nir2 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25357 Product name: Recombinant human PITPNM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1158-1243 aa of BC022230 Sequence: LSDGYVAHLGQLEAGSHSHASSGPPRAALGKSSYGVAAPVDFLRKQSQLLRSRGPSQAEREGPGTPPTTLARGKARSISLKLDSEE 相同性解析による交差性が予測される生物種 |
| Full Name | phosphatidylinositol transfer protein, membrane-associated 1 |
| Calculated molecular weight | 135 kDa |
| Observed molecular weight | 150-160 kDa |
| GenBank accession number | BC022230 |
| Gene Symbol | Nir2 |
| Gene ID (NCBI) | 9600 |
| RRID | AB_2880712 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00562 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for Nir2 antibody 26983-1-AP | Download protocol |
| WB protocol for Nir2 antibody 26983-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Virol Nir2 Is an Effector of VAPs Necessary for Efficient Hepatitis C Virus Replication and Phosphatidylinositol 4-Phosphate Enrichment at the Viral Replication Organelle.
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Biochim Biophys Acta Mol Cell Res The role of Nir2, a lipid-transfer protein, in regulating endothelial cell functions |
