Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, NCCIT cells, HT-29 cells |
| Positive IHC detected in | mouse brain tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IF | See 3 publications below |
Product Information
67132-1-Ig targets Ninein in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, canine samples.
| Tested Reactivity | human, mouse, canine |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14652 Product name: Recombinant human Ninein protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1870-2020 aa of BC034708 Sequence: VRQLESNLLPKHQKHLNPSGTMNPTEQEKLSLKRECDQFQKEQSPANRKVSQMNSLEQELETIHLENEGLKKKQVKLDEQLMEMQHLRSTATPSPSPHAWDLQLLQQQACPMVPREQFLQLQRQLLQAERINQHLQEELENRTSETNTPQ 相同性解析による交差性が予測される生物種 |
| Full Name | ninein (GSK3B interacting protein) |
| Calculated molecular weight | 2090 aa, 243 kDa |
| Observed molecular weight | 220 kDa |
| GenBank accession number | BC034708 |
| Gene Symbol | Ninein |
| Gene ID (NCBI) | 51199 |
| RRID | AB_2882431 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8N4C6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ninein is a large coiled-coil protein that associates with the subdistal appendages of the mother centriole and the minus-ends of both centrioles. Ninein interacts with γ-tubulin and microtubule nucleation and anchoring at the centrosomes.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Ninein antibody 67132-1-Ig | Download protocol |
| IHC protocol for Ninein antibody 67132-1-Ig | Download protocol |
| WB protocol for Ninein antibody 67132-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Dev Cell Proteomic profiling of centrosomes across multiple mammalian cell and tissue types by an affinity capture method
| ||
Elife Trisomy 21 induces pericentrosomal crowding delaying primary ciliogenesis and mouse cerebellar development |