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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Noggin Monoclonal antibody

Noggin Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A11C1

Cat no : CL488-69010

Synonyms

NOG, noggin, SYM1, SYNS1

Validation Data Gallery

Applications:
  • FC experiment of PC-3 using CL488-69010

    FC experiment of PC-3 using CL488-69010

    1X10^6 PC-3 cells were stained with 0.5 ug CoraLite® Plus 488-conjugated Noggin antibody (CL488-69010, Clone: 2A11C1) (green), or 0.5 ug isotype control (black). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

Tested Applications

Positive FC detected inPC-3 cells
For other applications, we recommend the unconjugated version of this antibody, 69010-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-69010 targets Noggin in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Noggin fusion protein HZ-1118 相同性解析による交差性が予測される生物種
Full Name noggin
Gene symbol NOG
Gene ID (NCBI) 9241
RRIDAB_2919566
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Noggin is an extracellular polypeptide acting as an antagonist of bone morphogenetic proteins (BMPs) regulating embryonal development. Noggin inhibits activity of BMP-2, -4, -7, -13, and -14. Noggin is present extracellularly in the matrix or retained at the cell surface via interaction with heparin sulfate proteoglycans. In early development stages, Noggin is produced by the Spemann organizer, allowing dorsal-ventral patterning of BMPs (PMID: 8752214). Subsequently, Noggin is expressed by the notochord regulating BMP-4 signaling in neurogenesis. Additionally, Noggin is present during development in the dermal papilla, connective tissue of the hair follicle, lens, retina, and periocular mesenchyme, as well as in the mesoderm lineage regulating development of the bone, cartilage, and muscles.

This antibody is used to Block/Neutralize Noggin protein.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Noggin antibody CL488-69010Download protocol
Standard Protocols
Click here to view our Standard Protocols