CoraLite®555-conjugated NeutraKine® IFN Gamma Monoclonal antibody
NeutraKine® IFN Gamma Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
FC (Intra)
Conjugate
CoraLite®555 Fluorescent Dye
CloneNo.
1E10G7
Cat no : CL555-69007
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC detected in | PMA and ionomycin treated Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL555-69007 targets NeutraKine® IFN Gamma in FC (Intra) applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | NeutraKine® IFN Gamma fusion protein HZ-1301 相同性解析による交差性が予測される生物種 |
| Full Name | IFN gamma |
| Gene symbol | IFNG |
| Gene ID (NCBI) | 3458 |
| Conjugate | CoraLite®555 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 557 nm / 570nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Interferon gamma (IFNG) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.
This antibody can be used to neutralize the bioactivity of Interferon gamma.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL555 NeutraKine® IFN Gamma antibody CL555-69007 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
