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Validation Data Gallery

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  • Western Blot (WB) analysis of U-87 MG cells using Neuropilin 2 Polyclonal antibody (27193-1-AP)

    WB analysis of U-87 MG using 27193-1-AP (same clone as 27193-1-PBS)

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 27193-1-AP (Neuropilin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27193-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of U-87 MG cells using Neuropilin 2 Polyclonal antibody (27193-1-AP)

    IP experiment of U-87 MG using 27193-1-AP (same clone as 27193-1-PBS)

    IP result of anti-Neuropilin 2 (IP:27193-1-AP, 4ug; Detection:27193-1-AP 1:500) with U-87 MG cells lysate 760 ug. This data was developed using the same antibody clone with 27193-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using Neuropilin 2 Polyclonal antibody (27193-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 27193-1-AP (same clone as 27193-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27193-1-AP (Neuropilin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27193-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using Neuropilin 2 Polyclonal antibody (27193-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 27193-1-AP (same clone as 27193-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27193-1-AP (Neuropilin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27193-1-PBS in a different storage buffer formulation.

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Neuropilin 2 Polyclonal antibody, PBS Only

Neuropilin 2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 27193-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

NRP2, Neuropilin-2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27193-1-PBS targets Neuropilin 2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Neuropilin 2 fusion protein Ag25622 相同性解析による交差性が予測される生物種
Full Name neuropilin 2
Observed molecular weight120-130 kDa
GenBank accession numberBC104770
Gene Symbol Neuropilin 2
Gene ID (NCBI) 8828
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60462
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Neuropilin 2 (NRP2) is a single-chain transmembrane glycoprotein that acts as a co-receptor of VEGF. NRP2 is mainly expressed in lymphatic and venous endothelial cells, and it is associated with the occurrence and development of several types of cancer. NRP2 is known to play a role in cardiovascular development, axon guidance, tumorigenesis, inflammation and cardiovascular disease.