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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Neuropilin 1/CD304 Recombinant antibody (84429-5-RR)

    WB analysis using 84429-5-RR (same clone as 84429-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84429-5-RR (NRP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84429-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Neuropilin 1/CD304 Recombinant antibody (84429-5-RR)

    IHC staining of human placenta using 84429-5-RR (same clone as 84429-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84429-5-RR (Neuropilin 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84429-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Neuropilin 1/CD304 Recombinant antibody (84429-5-RR)

    IHC staining of human placenta using 84429-5-RR (same clone as 84429-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84429-5-RR (Neuropilin 1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84429-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using Neuropilin 1/CD304 Recombinant antibody (84429-5-RR)

    IF Staining of PC-3 using 84429-5-RR (same clone as 84429-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using Neuropilin 1 antibody (84429-5-RR, Clone: 241812G11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84429-5-PBS in a different storage buffer formulation.

Neuropilin 1/CD304 Recombinant monoclonal antibody, PBS Only

Neuropilin 1/CD304 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84429-5-PBS
Clone No.241812G11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

neuropilin 1, NRP1, 241812G11, BDCA4, CD304

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84429-5-PBS targets Neuropilin 1/CD304 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg2331

Product name: Recombinant Human Neuropilin-1 protein (rFc Tag)(HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 22-644 aa of BC007533

Sequence: FRNDKCGDTIKIESPGYLTSPGYPHSYHPSEKCEWLIQAPDPYQRIMINFNPHFDLEDRDCKYDYVEVFDGENENGHFRGKFCGKIAPPPVVSSGPFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTTPSGVIKSPGFPEKYPNSLECTYIVFVPKMSEIILEFESFDLEPDSNPPGGMFCRYDRLEIWDGFPDVGPHIGRYCGQKTPGRIRSSSGILSMVFYTDSAIAKEGFSANYSVLQSSVSEDFKCMEALGMESGEIHSDQITASSQYSTNWSAERSRLNYPENGWTPGEDSYREWIQVDLGLLRFVTAVGTQGAISKETKKKYYVKTYKIDVSSNGEDWITIKEGNKPVLFQGNTNPTDVVVAVFPKPLITRFVRIKPATWETGISMRFEVYGCKITDYPCSGMLGMVSGLISDSQITSSNQGDRNWMPENIRLVTSRSGWALPPAPHSYINEWLQIDLGEEKIVRGIIIQGGKHRENKVFMRKFKIGYSNNGSDWKMIMDDSKRKAKSFEGNNNYDTPELRTFPALSTRFIRIYPERATHGGLGLRMELLGCEVEAPTAGPTTPNGNLVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTVIDSTIQSGIK

相同性解析による交差性が予測される生物種
Full Name neuropilin 1
Observed molecular weight130-140 kDa
GenBank accession numberBC007533
Gene Symbol Neuropilin 1
Gene ID (NCBI) 8829
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDO14786
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Neuropilin-1 (NRP1) is a 130-140 kDa transmembrane glycoprotein expressed by endothelial, dendritic, and regulatory T cells, as well as several other normal cell types and malignant tumor cells. NRP1 was first identified as a semaphorin (SEMA) receptor, involved in axonal guidance in embryonic development. NRP1 was also shown to act as a receptor for vascular endothelial growth factor (VEGF) and a promoter of angiogenesis through its interaction with VEGF-A165 (and other VEGFs) and the receptor tyrosine kinase (RTK) VEGF-R2. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.

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