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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NeuN Recombinant antibody (84401-4-RR)

    WB analysis using 84401-4-RR (same clone as 84401-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84401-4-RR (NeuN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NeuN Recombinant antibody (84401-4-RR)

    WB analysis using 84401-4-RR (same clone as 84401-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84401-4-RR (NeuN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using NeuN Recombinant antibody (84401-4-RR)

    IHC staining of mouse cerebellum using 84401-4-RR (same clone as 84401-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84401-4-RR (NeuN antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using NeuN Recombinant antibody (84401-4-RR)

    IHC staining of mouse cerebellum using 84401-4-RR (same clone as 84401-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84401-4-RR (NeuN antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using NeuN Recombinant antibody (84401-4-RR)

    IF Staining of rat brain using 84401-4-RR (same clone as 84401-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NeuN antibody (84401-4-RR, Clone: 241461G7 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), GFAP antibody (60190-1-Ig, Clone: 4B2E10, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using NeuN Recombinant antibody (84401-4-RR)

    IF Staining of mouse cerebellum using 84401-4-RR (same clone as 84401-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NeuN antibody (84401-4-RR, Clone: 241461G7 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using NeuN Recombinant antibody (84401-4-RR)

    IF Staining of mouse brain using 84401-4-RR (same clone as 84401-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NeuN antibody (84401-4-RR, Clone: 241461G7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using NeuN Recombinant antibody (84401-4-RR)

    IF Staining of rat brain using 84401-4-RR (same clone as 84401-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NeuN antibody (84401-4-RR, Clone: 241461G7 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), GFAP antibody (60190-1-Ig, Clone: 4B2E10, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using NeuN Recombinant antibody (84401-4-RR)

    IF Staining of mouse brain using 84401-4-RR (same clone as 84401-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using NeuN antibody (84401-4-RR, Clone: 241461G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84401-4-RR

    Affinity and Kinetic Characterization of 84401-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84401-4-RR against Human NeuN were performed. The affinity constant is 0.986 nM.

NeuN Recombinant monoclonal antibody, PBS Only

NeuN Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, IF-Fro, Indirect ELISA
Cat No. 84401-4-PBS
Clone No.241461G7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, IF-Fro, Indirect ELISA

Synonyms

241461G7, Fox-1 homolog C, FOX3, HRNBP3, NeuN antigen

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84401-4-PBS targets NeuN in WB, IHC, IF-P, IF-Fro, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag25689

Product name: Recombinant human NeuN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-100 aa of NM_001082575

Sequence: MAQPYPPAQYPPPPQNGIPAEYAPPPPHPTQDYSGQTPVPTEHGMTLYTPAQTHPEQPGSEASTQPIAGTQTVPQTDEAAQTDSQPLHPSDPTEKQQPKR

相同性解析による交差性が予測される生物種
Full Name hexaribonucleotide binding protein 3
Observed molecular weight 46-52 kDa
GenBank accession numberNM_001082575
Gene Symbol NeuN
Gene ID (NCBI) 146713
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDA6NFN3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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