Validation Data Gallery

Filter: AllWBIHCIF-PIF-FroFC (Intra)

WB analysis using 26975-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26975-1-AP (NeuN antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours.
WB analysis using 26975-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26975-1-AP (NeuN antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
WB analysis using 26975-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26975-1-AP (NeuN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of mouse cerebellum using 26975-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 26975-1-AP (NeuN antibody) at dilution of 1:30000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat cerebellum using 26975-1-AP
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 26975-1-AP (NeuN antibody) at dilution of 1:30000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat cerebellum using 26975-1-AP
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 26975-1-AP (NeuN antibody) at dilution of 1:30000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of rat brain using 26975-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NeuN antibody (26975-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), GFAP antibody (60190-1-Ig, Clone: 4B2E10, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of rat cerebellum using 26975-1-AP
Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using 26975-1-AP (NeuN antibody, green), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, red).
IF Staining of mouse cerebellum using 26975-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NeuN antibody (26975-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse cerebellum using 26975-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using NeuN antibody (26975-1-AP) at dilution of 1:5000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of rat cerebellum using 26975-1-AP
Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using 26975-1-AP (NeuN antibody, green), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, red).
IF Staining of rat brain using 26975-1-AP
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 26975-1-AP (NeuN antibody), at dilution of 1:100 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 66375-1-Ig (TUBB3 antibody, green).
IF Staining of rat cerebellum using 26975-1-AP
Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using 26975-1-AP (NeuN antibody, green), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, red).
IF Staining of mouse brain using 26975-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using NeuN antibody (26975-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-4).
FC experiment of U-87 MG using 26975-1-AP
1X10^6 U-87 MG cells were intracellularly stained with 0.2 ug Anti-Human NeuN (26975-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

NeuN Polyclonal antibody

NeuN Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

Cat No. 26975-1-AP

Publications(361)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (2)

Applications

WB, IHC, IF-P, IF-Fro, FC (Intra), Dot blot, ELISA

Synonyms

Fox-1 homolog C, FOX3, HRNBP3, NeuN antigen, Neuronal nuclei antigen

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®555 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (361)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, rat brain tissue, mouse cerebellum tissue, rat cerebellum tissue
Positive IHC detected in	mouse cerebellum tissue, rat cerebellum tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	rat brain tissue, rat cerebellum tissue, mouse cerebellum tissue
Positive IF-Fro detected in	mouse brain tissue
Positive FC (Intra) detected in	U-87 MG cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:20000-1:100000
Immunohistochemistry (IHC)	IHC : 1:5000-1:30000
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Immunofluorescence (IF)-FRO	IF-FRO : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 50 publications below
IHC	See 54 publications below
IF	See 288 publications below

Product Information

26975-1-AP targets NeuN in WB, IHC, IF-P, IF-Fro, FC (Intra), Dot blot, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Cited Reactivity	human, mouse, rat, pig, monkey, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag25689 Product name: Recombinant human NeuN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-100 aa of NM_001082575 Sequence: MAQPYPPAQYPPPPQNGIPAEYAPPPPHPTQDYSGQTPVPTEHGMTLYTPAQTHPEQPGSEASTQPIAGTQTVPQTDEAAQTDSQPLHPSDPTEKQQPKR 相同性解析による交差性が予測される生物種
Full Name	hexaribonucleotide binding protein 3
Observed molecular weight	46-52 kDa
GenBank accession number	NM_001082575
Gene Symbol	NeuN
Gene ID (NCBI)	146713
RRID	AB_2880708
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	A6NFN3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Function

NeuN, also known as FOX3 or RBFOX3, belongs to a family of tissue-specific splicing regulators and is involved in neural circuitry balance, as well as neurogenesis and synaptogenesis (PMID: 26619789).

Tissue specificity

NeuN is exclusively present in post-mitotic neurons and is absent from neural progenitors, oligodendrocytes, astrocytes, and glia (PMID: 1483388).

Involvement in disease

· NeuN cytoplasmic localization is increased in the neurons of patients with HIV-associated neurocognitive disorders (PMID: 24215932).

Isoforms

There are 4 isoforms of NeuN, migrating in the 45-50 kDa range (PMID: 21747913).

Post-translational modifications

Currently not known.

Cellular localization

NeuN predominantly localizes to the nucleus but can also be present in the cytoplasm. Isoforms of NeuN differ in their cytoplasmic/nucleus localization (PMID: 21747913).

Protocols

Product Specific Protocols
IF protocol for NeuN antibody 26975-1-AP	Download protocol
IHC protocol for NeuN antibody 26975-1-AP	Download protocol
WB protocol for NeuN antibody 26975-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Immunity Fate mapping of Spp1 expression reveals age-dependent plasticity of disease-associated microglia-like cells after brain injury Authors - Yangning Lan View Article
mouse	IF	Adv Sci (Weinh) GPR37 Activation Alleviates Bone Cancer Pain via the Inhibition of Osteoclastogenesis and Neuronal Hyperexcitability Authors - Kaiyuan Wang View Article
mouse	IF	Brain Behav Immun Egln3 expression in microglia enhances the neuroinflammatory responses in Alzheimer's disease Authors - Jiaxin Guan View Article
mouse	IF	Neuron Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory. Authors - Xiaohui Li View Article
mouse	IF	Sci Adv CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome. Authors - Sefan Asamitsu View Article
human	IF	Cell Death Differ Redox regulation of TRIM28 facilitates neuronal ferroptosis by promoting SUMOylation and inhibiting OPTN-selective autophagic degradation of ACSL4 Authors - Wei Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 26975-1-AP

WB analysis using 26975-1-AP

WB analysis using 26975-1-AP

IHC staining of mouse cerebellum using 26975-1-AP

IHC staining of rat cerebellum using 26975-1-AP

IHC staining of rat cerebellum using 26975-1-AP

IF Staining of rat brain using 26975-1-AP

IF Staining of rat cerebellum using 26975-1-AP

IF Staining of mouse cerebellum using 26975-1-AP

IF Staining of mouse cerebellum using 26975-1-AP

IF Staining of rat cerebellum using 26975-1-AP

IF Staining of rat brain using 26975-1-AP

IF Staining of rat cerebellum using 26975-1-AP

IF Staining of mouse brain using 26975-1-AP

FC experiment of U-87 MG using 26975-1-AP

NeuN Polyclonal antibody

NeuN Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Fate mapping of Spp1 expression reveals age-dependent plasticity of disease-associated microglia-like cells after brain injury

GPR37 Activation Alleviates Bone Cancer Pain via the Inhibition of Osteoclastogenesis and Neuronal Hyperexcitability

Egln3 expression in microglia enhances the neuroinflammatory responses in Alzheimer's disease

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.

Redox regulation of TRIM28 facilitates neuronal ferroptosis by promoting SUMOylation and inhibiting OPTN-selective autophagic degradation of ACSL4