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Validation Data Gallery

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  • Western Blot (WB) analysis of Neuro-2a cells using Nestin Monoclonal antibody (66259-1-Ig)

    WB analysis of Neuro-2a using 66259-1-Ig (same clone as 66259-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66259-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Nestin Monoclonal antibody (66259-1-Ig)

    WB analysis of HEK-293 using 66259-1-Ig (same clone as 66259-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66259-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using Nestin Monoclonal antibody (66259-1-Ig)

    WB analysis of PC-12 using 66259-1-Ig (same clone as 66259-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66259-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat cerebellum tissue using Nestin Monoclonal antibody (66259-1-Ig)

    WB analysis of rat cerebellum using 66259-1-Ig (same clone as 66259-1-PBS)

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66259-1-PBS in a different storage buffer formulation.

Nestin Monoclonal antibody, PBS Only

Nestin Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66259-1-PBS
Clone No.1D11H2

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

NES, Nestin

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66259-1-PBS targets Nestin in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name nestin
Calculated molecular weight 177 kDa
Observed molecular weight 150-170 kDa
GenBank accession numberNM_006617
Gene Symbol Nestin
Gene ID (NCBI) 10763
RRIDAB_2756523
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP48681
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.

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