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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Nestin Polyclonal antibody (19483-1-AP)

    WB analysis using 19483-1-AP (same clone as 19483-1-PBS)

    Mouse brain lysates were subjected to SDS PAGE followed by western blot with 19483-1-AP (Nestin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19483-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using Nestin Polyclonal antibody (19483-1-AP)

    WB analysis of rat brain using 19483-1-AP (same clone as 19483-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 19483-1-AP (NES antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19483-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Nestin Polyclonal antibody (19483-1-AP)

    IHC staining of human gliomas using 19483-1-AP (same clone as 19483-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19483-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Nestin Polyclonal antibody (19483-1-AP)

    IHC staining of human gliomas using 19483-1-AP (same clone as 19483-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19483-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Nestin Polyclonal antibody (19483-1-AP)

    IHC staining of human kidney using 19483-1-AP (same clone as 19483-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 19483-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Nestin Polyclonal antibody (19483-1-AP)

    IHC staining of human kidney using 19483-1-AP (same clone as 19483-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 19483-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using Nestin Polyclonal antibody (19483-1-AP)

    IF Staining of mouse kidney using 19483-1-AP (same clone as 19483-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using Nestin antibody (19483-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19483-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using Nestin Polyclonal antibody (19483-1-AP)

    IF Staining of U-251 using 19483-1-AP (same clone as 19483-1-PBS)

    Immunofluorescent analysis of U-251 cells using 19483-1-AP (Nestin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19483-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Nestin Polyclonal antibody (19483-1-AP)

    IF Staining of SH-SY5Y using 19483-1-AP (same clone as 19483-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Nestin antibody (19483-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19483-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Nestin Polyclonal antibody (19483-1-AP)

    IF Staining of SH-SY5Y using 19483-1-AP (same clone as 19483-1-PBS)

    Immunofluorescent analysis of SH-SY5Y cells using 19483-1-AP (Nestin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19483-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using Nestin Polyclonal antibody (19483-1-AP)

    FC experiment of SH-SY5Y using 19483-1-AP (same clone as 19483-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Nestin Polyclonal antibody (19483-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 19483-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19483-1-PBS targets Nestin in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name nestin
Calculated molecular weight 177 kDa
Observed molecular weight 170-180 kDa
GenBank accession numberNM_006617
Gene Symbol Nestin
Gene ID (NCBI) 10763
RRIDAB_10644440
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48681
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 220-240 kDa and 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924).