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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using Nesprin 2 Polyclonal antibody (25265-1-AP)

    WB analysis of HEK-293 using 25265-1-AP (same clone as 25265-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25265-1-AP (SYNE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25265-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Nesprin 2 Polyclonal antibody (25265-1-AP)

    IF Staining of mouse brain using 25265-1-AP (same clone as 25265-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Nesprin 2 antibody (25265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25265-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Nesprin 2 Polyclonal antibody (25265-1-AP)

    IF Staining of mouse brain using 25265-1-AP (same clone as 25265-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Nesprin 2 antibody (25265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25265-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using Nesprin 2 Polyclonal antibody (25265-1-AP)

    IF Staining of rat brain using 25265-1-AP (same clone as 25265-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Nesprin 2 antibody (25265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25265-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Human dorsal cerebral organoid using Nesprin 2 Polyclonal antibody (25265-1-AP)

    IF Staining of Human dorsal cerebral organoid using 25265-1-AP (same clone as 25265-1-PBS)

    Human dorsal cerebral organoid at 12 weeks. 4% PFA fixed. Antibody dilution was 1:500 for Syne-2 (25265-1-AP; white), DAPI 1:1000. Image created by Wimmer Ryszard from the Institut Curie in Paris, France This data was developed using the same antibody clone with 25265-1-PBS in a different storage buffer formulation.

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Nesprin 2 Polyclonal antibody, PBS Only

Nesprin 2 Polyclonal Antibody for WB, IF, Indirect ELISA
Cat No. 25265-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25265-1-PBS targets Nesprin 2 in WB, IF, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name spectrin repeat containing, nuclear envelope 2
Calculated molecular weight 799 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberNM_015180
Gene Symbol Nesprin 2
Gene ID (NCBI) 23224
RRIDAB_2879995
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WXH0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Nesprin-2 is a member of actin-binding proteins that localize to the outer nuclear membrane. As a nuclear envelope component it provides a link between cytoskeletal components of the cytoplasm and the nucleoplasm. Nesprin genes generate, through alternative splicing and alternative promoter and/or terminator usage, a multitude of differentially expressed isoforms, differing in domain architecture and length. The largest protein isoform, known as NUANCE or GIANT, has a predicted MW around 796 kDa. Other isoforms range from 30 kDa to 119 kDa.