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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Nephrin Polyclonal antibody

Nephrin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22912-1-AP

Synonyms

CNF, Nephrin, NPHS1

Validation Data Gallery

Applications:
  • WB analysis using 22912-1-AP

    WB analysis using 22912-1-AP

    mouse kidney tissue lysates were subjected to SDS PAGE followed by western blot with 22912-1-AP (Nephrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat kidney using 22912-1-AP

    IHC staining of rat kidney using 22912-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 22912-1-AP (Nephrin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat kidney using 22912-1-AP

    IHC staining of rat kidney using 22912-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 22912-1-AP (Nephrin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse kidney tissue
Positive IHC detected inrat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

22912-1-AP targets Nephrin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Nephrin fusion protein Ag18994 相同性解析による交差性が予測される生物種
Full Name nephrosis 1, congenital, Finnish type (nephrin)
Calculated molecular weight 1241 aa, 135 kDa
Observed molecular weight120-130 kDa
GenBank accession numberBC156935
Gene symbol NPHS1
Gene ID (NCBI) 4868
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Podocytes are a major component of the glomerular blood filtration barrier. Podocyte protein Nephrin, also known as NPHS1, is the first to be identified as a central component of the slit diaphragm (SD), which is podocyte cell-cell junction. Mutations in Nephrin lead to congenital nephrosis, such as steroid-resistant proteinuria and congenital nephrotic syndrome of the Finnish type (CNF).

Protocols

Product Specific Protocols
WB protocol for Nephrin antibody 22912-1-APDownload protocol
IHC protocol for Nephrin antibody 22912-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols