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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Nectin-4/PVRL4 Polyclonal antibody (21903-1-AP)

    WB analysis using 21903-1-AP (same clone as 21903-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21903-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Nectin-4/PVRL4 Polyclonal antibody (21903-1-AP)

    IHC staining of human placenta using 21903-1-AP (same clone as 21903-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21903-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Nectin-4/PVRL4 Polyclonal antibody (21903-1-AP)

    IHC staining of human placenta using 21903-1-AP (same clone as 21903-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21903-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Nectin-4/PVRL4 Polyclonal antibody (21903-1-AP)

    IF Staining of MCF-7 using 21903-1-AP (same clone as 21903-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Nectin-4/PVRL4 antibody (21903-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21903-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Nectin-4/PVRL4 Polyclonal antibody, PBS Only

Nectin-4/PVRL4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21903-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Nectin-4, PVRL4, Ig superfamily receptor LNIR, LNIR, nectin 4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21903-1-PBS targets Nectin-4/PVRL4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Nectin-4/PVRL4 fusion protein Ag14127 相同性解析による交差性が予測される生物種
Full Name poliovirus receptor-related 4
Calculated molecular weight 510 aa, 55 kDa
Observed molecular weight55-80 kDa, 43 kDa,
GenBank accession numberBC010423
Gene Symbol Nectin-4
Gene ID (NCBI) 81607
RRIDAB_2878941
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96NY8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Nectin-4, also known as PVRL4, is a type-I transmembrane glycoprotein that belongs to the nectin subfamily of immunoglobulin-like adhesion molecules that participate in Ca(2+)-independent cell-cell adhesion (PMID: 18703497). The extracellular domain of Nectin-4, which contains three immunoglobulin-like domains (V and two C2-type domains, VCC), can be proteolytically cleaved to release a soluble form. Nectin-4 interacts with afadin via its carboxyl-terminal cytoplasmic sequence and trans-interacts with nectin-1/PRR1 through V domain interaction (PMID: 11544254). It acts as a receptor for measles virus (PMID: 22048310). Nectin-4 is overexpressed in multiple human malignancies and the aberrant expression is correlated with cancer progression and poor prognostic (PMID: 35131876). Nectin-4 can be detected as a glycosylated form of 60-80 kDa and a deglycosylated form of 55-70 kDa. The 43 kDa band immunoprecipitated in the supernatant corresponds to the soluble Nectin-4 form. (PMID: 15784625).