BLI binding kinetics of a human IgE antibody. 20 nM human IgE antibody was immobilized using Nano-CaptureLigand human IgE, VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors. Human IgE is stably captured by the Nano-CaptureLigand with negligible dissociation.

BLI binding kinetics of human, mouse and rabbit antibodies. 20 nM of human, mouse and rabbit antibodies were immobilized using Nano-CaptureLigand human IgE, VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors. Only human IgE antibody is captured by the Nano-CaptureLigand with negligible dissociation.

Immobilization of the Nano-CaptureLigand (green) on Biosensor (white) for biolayer interferometry (BLI) assay followed by capture of an antibody (orange). The setup is ready for characterization of the antibodies’ binding kinetics and affinity to target (red), antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma.

ELISA capture of a human IgE antibody using Nano-CaptureLigand human IgE, VHH, biotinylated. 50 nM Nano-CaptureLigand human IgE, VHH, biotinylated was used for coating on an avidin-coated MaxiSorp plate. Human IgE antibody was titrated in a 1:2 dilution series and detected with a rabbit anti-human IgE primary antibody and an alkaline phosphatase-conjugated detection antibody.

Sandwich ELISA: Immobilization of the Nano-CaptureLigand on streptavidin coated microplates for ELISA. No biotinylation of the antibody is required. Coating of the streptavidin microplate with Nano-CaptureLigand (top left). Site-directed antibody immobilization via Nano-CaptureLigand (top right). Addition of sample including target (red, bottom left). Addition of detection and secondary antibody for read-out (bottom right).

Species reactivity of Secondary VHH.