Validation Data Gallery
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Tested Applications
| Positive WB detected in | NCI-H1299 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85355-1-RR targets NY-ESO-1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | cancer/testis antigen 1A |
| Calculated molecular weight | 18 kDa |
| Observed molecular weight | 20 kDa |
| GenBank accession number | NM_139250 |
| Gene Symbol | NY-ESO-1 |
| Gene ID (NCBI) | 246100 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P78358 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CTAG1A, also named as NY-ESO-1, CTAG, CTAG1, ESO1, LAGE2, LAGE2A, CTAG1B, LAGE2B and CT6.1, belongs to the CTAG family.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NY-ESO-1 antibody 85355-1-RR | Download protocol |
| WB protocol for NY-ESO-1 antibody 85355-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
