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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NXN Polyclonal antibody (16128-1-AP)

    WB analysis of HeLa using 16128-1-AP (same clone as 16128-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16128-1-AP (NXN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NXN Polyclonal antibody (16128-1-AP)

    WB analysis using 16128-1-AP (same clone as 16128-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16128-1-AP (NXN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using NXN Polyclonal antibody (16128-1-AP)

    WB analysis of human heart using 16128-1-AP (same clone as 16128-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 16128-1-AP (NXN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using NXN Polyclonal antibody (16128-1-AP)

    IP experiment of HeLa using 16128-1-AP (same clone as 16128-1-PBS)

    IP result of anti-NXN (IP:16128-1-AP, 4ug; Detection:16128-1-AP 1:500) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NXN Polyclonal antibody (16128-1-AP)

    IHC staining of human colon cancer using 16128-1-AP (same clone as 16128-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16128-1-AP (NXN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NXN Polyclonal antibody (16128-1-AP)

    IHC staining of human colon cancer using 16128-1-AP (same clone as 16128-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16128-1-AP (NXN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NXN Polyclonal antibody (16128-1-AP)

    IF Staining of HeLa using 16128-1-AP (same clone as 16128-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NXN antibody (16128-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NXN Polyclonal antibody, PBS Only

NXN Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16128-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16128-1-PBS targets NXN in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9113

Product name: Recombinant human NXN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-326 aa of BC009327

Sequence: ADVSLHRNPATLKLWNKYRISNIPSLIFLDATTGKVVCRNGLLVIRDDPEGLEFPWGPKPFREVIAGPLLRNNGQSLESSSLEGSHVGVYFSAHWCPPCRSLTRVLVESYRKIKEAGQNFEIIFVSADRSEESFKQYFSEMPWLAVPYTDEARRSRLNRLYGIQGIPTLIMLDPQGEVITRQGRVEVLNDEDCREFPWHPKPVLELSDSNAAQLNEGPCLVLFVDSEDDGESEAAKQLIQPIAEKIIAKYKAKEEEAPLLFFVAGEDDMTDSLRDYTNLPEAAPLLTILDMSARAKYVMDVEEITPAIVEAFVNDFLAEKLKPEPI

相同性解析による交差性が予測される生物種
Full Name nucleoredoxin
Calculated molecular weight 435 aa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC009327
Gene Symbol NXN
Gene ID (NCBI) 64359
RRIDAB_2158114
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6DKJ4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Nucleoredoxin (NRX/NXN), a relatively uncharacterized member of the TRX family protein, has recently been reported to regulate the Wnt/β-catenin pathway, which itself regulates cell fate and early development, in a redox-dependent manner(PMID:17567240). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver(PMID:9119370). It has 3 isoforms produced by alternative splicing. This antibody is specific to NXN.