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Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using NWD1 Polyclonal antibody (25025-1-AP)

    WB analysis of SH-SY5Y using 25025-1-AP (same clone as 25025-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25025-1-AP (NWD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25025-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using NWD1 Polyclonal antibody (25025-1-AP)

    WB analysis of HepG2 using 25025-1-AP (same clone as 25025-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25025-1-AP (NWD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25025-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using NWD1 Polyclonal antibody (25025-1-AP)

    WB analysis of mouse brain using 25025-1-AP (same clone as 25025-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25025-1-AP (NWD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25025-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NWD1 Polyclonal antibody (25025-1-AP)

    IF Staining of HepG2 using 25025-1-AP (same clone as 25025-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 25025-1-AP (NWD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25025-1-PBS in a different storage buffer formulation.

NWD1 Polyclonal antibody, PBS Only

NWD1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 25025-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

NACHT domain- and WD repeat-containing protein 1, NWD1 Ens

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25025-1-PBS targets NWD1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21754

Product name: Recombinant human NWD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1009-1358 aa of BC117698

Sequence: TGLTAVSHNGSYVYFPKIGDKNKVTIWDLAEGEEQDSLDTSSEIRCLEVAEQRKLLFTGLVSGVVLVFPLNSRQDVICIPPPEARKAINCMSLSKCEDRLAIAYDNIVLVLDITSGDPCPVIDGPRYTFYTQLPETLSSVAILTDYRVVYSMTNGDLFLYECATSKAFPLETHRSRVACVEVSHKEQLVVSGSEDALLCLWDLQARKWKFEMSYTSSYCRGVQCACFSKDDKYVYVGLKDRSILVWSVLDGTLLTVQFVHAVVNRIIPTTSGFIAPTRHGYLIRENFQCLSAKASPQDPLKNFKKAMWMVKSRQREELVAAAGAPQDLESESAQGNETKSNKCSQVCLIV

相同性解析による交差性が予測される生物種
Full Name NACHT and WD repeat domain containing 1
Calculated molecular weight 1564 aa, 175 kDa
Observed molecular weight 77 kDa
GenBank accession numberBC117698
Gene Symbol NWD1
Gene ID (NCBI) 284434
RRIDAB_2879855
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ149M9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NWD1 also named as NWD1 Ens or NACHT and WD repeat domain containing 1 is a 1564 amino acid protein, which contains 1 NACHT domain and 13 WD repeats. The molecular weight of NWD1 was detected by Western blot. The result shows that the molecular weight of NWD1 is 77 kDa, but NWD1 is a 174 kDa protein according to UniProt. The function of NWD1 is still non-known, meanwhile the molecular weight of NWD1 is still remained to be confirmed.

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