Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68399-1-Ig targets NVL in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9321 Product name: Recombinant human NVL protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 309-659 aa of BC012105 Sequence: LPSKGVQEERLGTEPTSETQDELQRLLGLLRDQDPLSEEQMQGLCIELNDFIVALSSVQPSAKREGFVTVPNVTWADIGALEDIREELTMAILAPVRNPDQFKALGLVTPAGVLLAGPPGCGKTLLAKAVANESGLNFISVKGPELLNMYVGESERAVRQVFQRAKNSAPCVIFFDEVDALCPRRSDRETGASVRVVNQLLTEMDGLEARQQVFIMAATNRPDIIDPAILRPGRLDKTLFVGLPPPADRLAILKTITKNGTKPPLDADVNLEAIAGDLRCDCYTGADLSALVREASICALRQEMARQKSGNEKGELKVSHKHFEEAFKKVRSSISKKDQIMYERLQESLSR 相同性解析による交差性が予測される生物種 |
| Full Name | nuclear VCP-like |
| Calculated molecular weight | 856aa,95 kDa; 659aa,73 kDa |
| Observed molecular weight | 95 kDa |
| GenBank accession number | BC012105 |
| Gene Symbol | NVL |
| Gene ID (NCBI) | 4931 |
| RRID | AB_3085116 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O15381 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NVL(nuclear VCP-like) , also named as NVL2, belongs to the AAA ATPase family and may facilitate the dissociation and recycling of nucleolin, thereby promoting efficient ribosome biogenesis. It acts as an ATP-dependent zinc metallopeptidase. NVL plays an essential role in ribosome biogenesis by specifically associating with ribosomal protein L5 and modulating the function of DOB1, thereby participating in the assembly of the 60S ribosomal subunit. Additionally, NVL is required for telomerase assembly and the regulation of telomerase activity, and is involved in pre-rRNA processing. This protein has 5 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for NVL antibody 68399-1-Ig | Download protocol |
| WB protocol for NVL antibody 68399-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |