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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using NUPL2 Polyclonal antibody (16587-1-AP)

    WB analysis of HEK-293 using 16587-1-AP (same clone as 16587-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16587-1-AP (NUPL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16587-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using NUPL2 Polyclonal antibody (16587-1-AP)

    WB analysis of human liver using 16587-1-AP (same clone as 16587-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 16587-1-AP (NUPL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16587-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using NUPL2 Polyclonal antibody (16587-1-AP)

    WB analysis of L02 using 16587-1-AP (same clone as 16587-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 16587-1-AP (NUPL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16587-1-PBS in a different storage buffer formulation.

NUPL2 Polyclonal antibody, PBS Only

NUPL2 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 16587-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16587-1-PBS targets NUPL2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8816

Product name: Recombinant human NUPL2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-224 aa of BC005327

Sequence: MAICQFFLQGRCRFGDRCWNEHPGARGAGGGRQQPQQQPSGNNRRGWNTTSQRYSNVIQPSSFSKSTPWGGSRDQEKPYFSSFDSGASTNRKEGFGLSENPFASLSPDEQKDEKKLLEGIVKDMEVWESSGQWMFSVYSPVKKKPNISGFTDISPEELRLEYHNFLTSNNLQSYLNSVQRLINQWRNRVNELKSLNISTKVALKCLWKIYTHLSPKIYGPDCAL

相同性解析による交差性が予測される生物種
Full Name nucleoporin like 2
Calculated molecular weight 45 kDa
Observed molecular weight 45 kDa, 33-36 kDa
GenBank accession numberBC005327
Gene Symbol NUPL2
Gene ID (NCBI) 11097
RRIDAB_2267702
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO15504
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NUPL2 (also known as NLP-1, or hCG1) is an ubiquitous nuclear protein containing many phenylalanine-glycine (FG) repeats. As the functional orthologue of the budding yeast Nup42/Rip1 nucleoporin, NUPL2 is required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm (PMID: 10610322). In case of infection by HIV-1, it may participate in the docking of viral Vpr at the nuclear envelope (PMID: 16000379). NUPL2 localizes to the nuclear envelope and could also be mobile within the nucleus. It promotes CRM1-dependent nuclear protein export (PMID: 22250199).

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