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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NUP62CL Polyclonal antibody (25381-1-AP)

    WB analysis of HeLa using 25381-1-AP (same clone as 25381-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 25381-1-AP (NUP62CL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25381-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using NUP62CL Polyclonal antibody (25381-1-AP)

    WB analysis of Jurkat using 25381-1-AP (same clone as 25381-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25381-1-AP (NUP62CL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25381-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using NUP62CL Polyclonal antibody (25381-1-AP)

    WB analysis of SH-SY5Y using 25381-1-AP (same clone as 25381-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25381-1-AP (NUP62CL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25381-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NUP62CL Polyclonal antibody (25381-1-AP)

    IHC staining of human lung cancer using 25381-1-AP (same clone as 25381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25381-1-AP (NUP62CL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25381-1-PBS in a different storage buffer formulation.

NUP62CL Polyclonal antibody, PBS Only

NUP62CL Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25381-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25381-1-PBS targets NUP62CL in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21107

Product name: Recombinant human NUP62CL protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-72 aa of BC017799

Sequence: MQFTSISNSLTSTAAIGLSFTTSTTTTATFTTNTTTTITSGFTVNQNQLLSRGFENLVPYTSTVRFVFYMEK

相同性解析による交差性が予測される生物種
Full Name nucleoporin 62kDa C-terminal like
Calculated molecular weight 72 aa, 8 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC017799
Gene Symbol NUP62CL
Gene ID (NCBI) 54830
RRIDAB_2880051
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H1M0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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