Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 16232-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16232-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 16232-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 16232-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 16232-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16232-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human stomach using 16232-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16232-1-AP (NUP54 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 16232-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16232-1-AP (NUP54 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

NUP54 Polyclonal antibody

NUP54 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16232-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Nucleoporin p54, nucleoporin 54kDa, 54 kDa nucleoporin

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, K-562 cells, mouse liver tissue, HepG2 cells, mouse heart tissue, A549 cells
Positive IHC detected in	human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:300-1:2000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 5 publications below

Product Information

16232-1-AP targets NUP54 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8882 Product name: Recombinant human NUP54 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-351 aa of BC012559 Sequence: MAFNFGAPSGTSGTAAATAAPAGGFGGFGTTSTTAGSAFSFSAPTNTGTTGLFGGTQNKGFGFGTGFGTTTGTSTGLGTGLGTGLGFGGFNTQQQQQTTLGGLFSQPTQAPTQSNQLINTASALSAPTLLGDERDAILAKWNQLQAFWGTGKGYFNNNIPPVEFTQENPFCRFKAVGYSCMPSNKDEDGLVVLVFNKKETEIRSQQQQLVESLHKVLGGNQTLTVNVEGTKTLPDDQTEVVIYVVERSPNGTSRRVPATTLYAHFEQANIKTQLQQLGVTLSMTRTELSPAQIKQLLQNPPAGVDPIIWEQAKVDNPDSEKLIPVPMVGFKELLRRLKVQDQMTKQHQTRL 相同性解析による交差性が予測される生物種
Full Name	nucleoporin 54kDa
Calculated molecular weight	507 aa, 55 kDa
Observed molecular weight	55 kDa
GenBank accession number	BC012559
Gene Symbol	NUP54
Gene ID (NCBI)	53371
RRID	AB_10644484
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q7Z3B4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for NUP54 antibody 16232-1-AP	Download protocol
IHC protocol for NUP54 antibody 16232-1-AP	Download protocol
WB protocol for NUP54 antibody 16232-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Dev Cell Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination Authors - Li Li View Article
human	WB	Elife Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2. Authors - Melissa Kane View Article
human	WB	J Exp Clin Cancer Res Transketolase (TKT) activity and nuclear localization promote hepatocellular carcinoma in a metabolic and a non-metabolic manner. Authors - Zhaoyu Qin View Article
mouse	WB	Cell Rep Nucleoporin Seh1 maintains Schwann cell homeostasis by regulating genome stability and necroptosis Authors - Mei Wu View Article
human	WB	bioRxiv Global mapping of the Chlamydia trachomatis conventional secreted effector - host interactome reveals CebN interacts with nucleoporins and Rae1 to impede STAT1 nuclear translocation Authors - Brianna Steiert View Article
human	WB	Nat Phys Structural anisotropy results in mechano-directional transport of proteins across nuclear pores Authors - Fani Panagaki KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 16232-1-AP

WB analysis of mouse liver using 16232-1-AP

WB analysis of mouse heart using 16232-1-AP

WB analysis of K-562 using 16232-1-AP

WB analysis of A549 using 16232-1-AP

WB analysis of HepG2 using 16232-1-AP

IHC staining of human stomach using 16232-1-AP

IF Staining of HepG2 using 16232-1-AP

NUP54 Polyclonal antibody

NUP54 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination

Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.

Transketolase (TKT) activity and nuclear localization promote hepatocellular carcinoma in a metabolic and a non-metabolic manner.

Nucleoporin Seh1 maintains Schwann cell homeostasis by regulating genome stability and necroptosis

Global mapping of the Chlamydia trachomatis conventional secreted effector - host interactome reveals CebN interacts with nucleoporins and Rae1 to impede STAT1 nuclear translocation

Structural anisotropy results in mechano-directional transport of proteins across nuclear pores