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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUMB Recombinant antibody (85939-4-RR)

    WB analysis using 85939-4-RR (same clone as 85939-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85939-4-RR (NUMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85939-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NUMB Recombinant antibody (85939-4-RR)

    WB analysis using 85939-4-RR (same clone as 85939-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85939-4-RR (NUMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85939-4-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using NUMB Recombinant antibody (85939-4-RR)

    IP experiment of HeLa using 85939-4-RR (same clone as 85939-4-PBS)

    IP result of anti-NUMB (IP:85939-4-RR, 4ug; Detection:85939-4-RR 1:3000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 85939-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NUMB Recombinant antibody (85939-4-RR)

    IHC staining of human breast cancer using 85939-4-RR (same clone as 85939-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85939-4-RR (NUMB antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85939-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NUMB Recombinant antibody (85939-4-RR)

    IHC staining of human breast cancer using 85939-4-RR (same clone as 85939-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85939-4-RR (NUMB antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85939-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85939-4-RR

    Affinity and Kinetic Characterization of 85939-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85939-4-RR against Human NUMB were performed. The affinity constant is 1.15 nM.

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NUMB Recombinant antibody, PBS Only

NUMB Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 85939-4-PBS
Clone No.250417F6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

C14orf41, h Numb, h-Numb

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85939-4-PBS targets NUMB in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NUMB fusion protein Ag31553 相同性解析による交差性が予測される生物種
Full Name numb homolog (Drosophila)
Calculated molecular weight71KD
Observed molecular weight66-71 kDa
GenBank accession numberNM_001005743
Gene Symbol NUMB
Gene ID (NCBI) 8650
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49757
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NUMB, also named as S171, plays a role in the determination of cell fates during development. It is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The degradation of NUMB is induced in a proteasome-dependent manner by MDM2. Four transcript variants encoding different isoforms have been found for this gene.