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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using NUMB Monoclonal antibody (60137-2-Ig)

    WB analysis of A431 using 60137-2-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 60137-2-Ig (NUMB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using NUMB Monoclonal antibody (60137-2-Ig)

    WB analysis of A431 using 60137-2-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 60137-2-Ig (NUMB antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using NUMB Monoclonal antibody (60137-2-Ig)

    WB analysis of A431 using 60137-2-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 60137-2-Ig (NUMB antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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NUMB Monoclonal antibody

NUMB Monoclonal Antibody for ELISA
Cat No. 60137-2-Ig
Clone No.1A8A1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60137-2-Ig targets NUMB in ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen peptide 相同性解析による交差性が予測される生物種
Full Name numb homolog (Drosophila)
Calculated molecular weight 71 kDa
GenBank accession numberBC068476
Gene Symbol NUMB
Gene ID (NCBI) 8650
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49757
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NUMB, also named as S171, plays a role in the determination of cell fates during development. It is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The degradation of NUMB is induced in a proteasome-dependent manner by MDM2. Four transcript variants encoding different isoforms have been found for this gene. The antibody can bind to all these four isoforms P65, P66, P71 and P72, can’t recognize the NUMBL.