Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IF | See 3 publications below |
| CoIP | See 1 publications below |
Product Information
60137-1-Ig targets NUMB in WB, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | peptide 相同性解析による交差性が予測される生物種 |
| Full Name | numb homolog (Drosophila) |
| Calculated molecular weight | 71 kDa |
| Observed molecular weight | 66-72 kDa |
| GenBank accession number | BC068476 |
| Gene Symbol | NUMB |
| Gene ID (NCBI) | 8650 |
| RRID | AB_10598775 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P49757 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NUMB, also named as S171, plays a role in the determination of cell fates during development. It is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The degradation of NUMB is induced in a proteasome-dependent manner by MDM2. Four transcript variants encoding different isoforms have been found for this gene. The antibody can bind to all these four isoforms P65, P66, P71 and P72, can't recognize the NUMBL.
Publications
| Species | Application | Title |
|---|---|---|
Sci Rep Hepatic stem cell Numb gene is a potential target of Huang Qi Decoction against cholestatic liver fibrosis.
| ||
Neurosci Lett Numb deficiency causes impaired trafficking of mGlu5 in neurons and autistic-like behaviors.
| ||
Mol Biol Rep Different roles of Numb-p72 and Numb-p65 on the trafficking of metabotropic glutamate receptor 5. | ||
Stem Cell Res Ther BM-MSCs overexpressing the Numb enhance the therapeutic effect on cholestatic liver fibrosis by inhibiting the ductular reaction | ||
Cell Rep Stabilizing vimentin phosphorylation inhibits stem-like cell properties and metastasis of hybrid epithelial/mesenchymal carcinomas |