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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUDT15 Polyclonal antibody (25914-1-AP)

    WB analysis using 25914-1-AP

    Jurkat lysates were subjected to SDS PAGE followed by western blot with 25914-1-AP (NUDT15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NUDT15 Polyclonal antibody (25914-1-AP)

    WB analysis of HeLa using 25914-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25914-1-AP (NUDT15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

NUDT15 Polyclonal antibody

NUDT15 Polyclonal Antibody for WB, ELISA
Cat No. 25914-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

8 oxo dGTPase NUDT15, EC:3.6.1.9, MTH2, MutT homolog 2, Nucleoside diphosphate-linked moiety X motif 15

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inJurkat cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25914-1-AP targets NUDT15 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag23242

Product name: Recombinant human NUDT15 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-164 aa of BC133015

Sequence: MTASAQPRGRRPGVGVGVVVTSCKHPRCVLLGKRKGSVGAGSFQLPGGHLEFGETWEECAQRETWEEAALHLKNVHFASVVNSFIEKENYHYVTILMKGEVDVTHDSEPKNVEPEKNESWEWVPWEELPPLDQLFWGLRCLKEQGYDPFKEDLNHLVGYKGNHL

相同性解析による交差性が予測される生物種
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 15
Observed molecular weight19 kDa
GenBank accession numberBC133015
Gene Symbol NUDT15
Gene ID (NCBI) 55270
RRIDAB_2880294
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NV35
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NUDT15 (nucleoside diphosphate-linked moiety X-type motif 15) is homologues to NUDT1 (MutT homologue 1, MTH1) and thus has previously been referred to as MTH2. NUDT15 is a key enzyme in thiopurine metabolism and tempers the activity of thiopurine drugs by catalyzing the hydrolysis of 6-thio-dGTP and 6-thio-GTP (PMID: 27530327, 32690945). NUDT15 is a 164-amino-acid protein that belongs to the nudix hydrolase enzyme family, whose members are capable of hydrolyzing compounds with the general structure of a nucleoside diphosphate linked to another moiety, X. (PMID: 25108385)

Protocols

Product Specific Protocols
WB protocol for NUDT15 antibody 25914-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols