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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUDT12 Polyclonal antibody (17487-1-AP)

    WB analysis using 17487-1-AP

    mouse liver tissues were subjected to SDS PAGE followed by western blot with 17487-1-AP (NUDT12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NUDT12 Polyclonal antibody (17487-1-AP)

    WB analysis of HeLa using 17487-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17487-1-AP (NUDT12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NUDT12 Polyclonal antibody (17487-1-AP)

    IF Staining of HeLa using 17487-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NUDT12 antibody (17487-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using NUDT12 Polyclonal antibody (17487-1-AP)

    IF Staining of MCF-7 using 17487-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NUDT12 antibody (17487-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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NUDT12 Polyclonal antibody

NUDT12 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17487-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

DeNADding enzyme NUDT12, EC:3.6.1.-, EC:3.6.1.22, NAD-capped RNA hydrolase NUDT12, NADH pyrophosphatase NUDT12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, HeLa cells
Positive IF/ICC detected inMCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17487-1-AP targets NUDT12 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUDT12 fusion protein Ag11557 相同性解析による交差性が予測される生物種
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 12
Calculated molecular weight 462 aa, 52 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC041099
Gene Symbol NUDT12
Gene ID (NCBI) 83594
RRIDAB_2236164
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQG2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NUDT12 is a member of the Nudix superfamily of pyrophosphohydrolases that has a broad range of substrates, including (d)NTPs, nucleotides, capped RNAs, dinucleotide coenzymes (NAD, FAD, dpCoA), and their derivatives. NUDT12 is active only as homodimers, with each monomer contributing to creation of the two functional catalytic pockets (PMID: 37301333, 31875550). The NUDT12 gene on chromosome 5q21 encodes the open reading frame of the putative 462-amino-acid, 52 kDa NUDT12 protein (PMID: 12790796).

Protocols

Product Specific Protocols
WB protocol for NUDT12 antibody 17487-1-APDownload protocol
IF protocol for NUDT12 antibody 17487-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

A tumor-specific modulation of heterogeneous ribonucleoprotein A0 promotes excessive mitosis and growth in colorectal cancer cells.

Authors - Hiroaki Konishi
View Article