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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse colon tissue using NUBPL Polyclonal antibody (17393-1-AP)

    WB analysis of mouse colon using 17393-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 17393-1-AP (NUBPL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using NUBPL Polyclonal antibody (17393-1-AP)

    WB analysis of COLO 320 using 17393-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 17393-1-AP (NUBPL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using NUBPL Polyclonal antibody (17393-1-AP)

    WB analysis of COLO 320 using 17393-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 17393-1-AP (NUBPL Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NUBPL Polyclonal antibody (17393-1-AP)

    IHC staining of human colon cancer using 17393-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17393-1-AP (NUBPL antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NUBPL Polyclonal antibody (17393-1-AP)

    IHC staining of human colon cancer using 17393-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17393-1-AP (NUBPL antibody) at dilution of 1:200 (under 40x lens).

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NUBPL Polyclonal antibody

NUBPL Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17393-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

C14orf127, huInd1, IND1 homolog, Iron sulfur protein NUBPL, NUBPL

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse colon tissue, COLO 320 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

17393-1-AP targets NUBPL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUBPL fusion protein Ag11291 相同性解析による交差性が予測される生物種
Full Name nucleotide binding protein-like
Calculated molecular weight289aa,31 kDa; 319aa,34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC024919
Gene Symbol NUBPL
Gene ID (NCBI) 80224
RRIDAB_2878402
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TB37
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NUBPL antibody 17393-1-APDownload protocol
IHC protocol for NUBPL antibody 17393-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Heliyon

Disulfidptosis and ferroptosis related genes define the immune microenvironment and NUBPL serves as a potential biomarker for predicting prognosis and immunotherapy response in bladder cancer

Authors - Xuezhou Zhang
View Article