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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NUBPL Polyclonal antibody

NUBPL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17393-1-AP

Synonyms

C14orf127, huInd1, IND1 homolog, Iron sulfur protein NUBPL, NUBPL

Validation Data Gallery

Applications:
  • WB analysis of mouse colon using 17393-1-AP

    WB analysis of mouse colon using 17393-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 17393-1-AP (NUBPL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of COLO 320 using 17393-1-AP

    WB analysis of COLO 320 using 17393-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 17393-1-AP (NUBPL Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of COLO 320 using 17393-1-AP

    WB analysis of COLO 320 using 17393-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 17393-1-AP (NUBPL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 17393-1-AP

    IHC staining of human colon cancer using 17393-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17393-1-AP (NUBPL antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon cancer using 17393-1-AP

    IHC staining of human colon cancer using 17393-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17393-1-AP (NUBPL antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inmouse colon tissue, COLO 320 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17393-1-AP is NUBPL Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUBPL fusion protein Ag11291 相同性解析による交差性が予測される生物種
Full Name nucleotide binding protein-like
Calculated molecular weight289aa,31 kDa; 319aa,34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC024919
Gene symbol NUBPL
Gene ID (NCBI) 80224
RRIDAB_2878402
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NUBPL antibody 17393-1-APDownload protocol
IHC protocol for NUBPL antibody 17393-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols