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Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using NTM Polyclonal antibody (14373-1-AP)

    WB analysis of SH-SY5Y using 14373-1-AP

    WB result of NTM antibody (14373-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NTM transfected SH-SY5Y cells.

  • Western Blot (WB) analysis of SH-SY5Y cells using NTM Polyclonal antibody (14373-1-AP)

    WB analysis of SH-SY5Y using 14373-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14373-1-AP (NTM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using NTM Polyclonal antibody (14373-1-AP)

    WB analysis of mouse brain using 14373-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14373-1-AP (NTM Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NTM Polyclonal antibody (14373-1-AP)

    IHC staining of mouse brain using 14373-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14373-1-AP (NTM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NTM Polyclonal antibody (14373-1-AP)

    IHC staining of mouse brain using 14373-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14373-1-AP (NTM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

NTM Polyclonal antibody

NTM Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14373-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

HNT, IgLON family member 2, IGLON2, neurotrimin, NT, NTM, NTRI

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14373-1-AP targets NTM in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5746

Product name: Recombinant human NTM protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 3-316 aa of BC050716

Sequence: VCGYLFLPWKCLVVVSLRLLFLVPTGVPVRSGDATFPKAMDNVTVRQGESATLRCTIDNRVTRVAWLNRSTILYAGNDKWCLDPRVVLLSNTQTQYSIEIQNVDVYDEGPYTCSVQTDNHPKTSRVHLIVQVSPKIVEISSDISINEGNNISLTCIATGRPEPTVTWRHISPKAVGFVSEDEYLEIQGITREQSGDYECSASNDVAAPVVRRVKVTVNYPPYISEAKGTGVPVGQKGTLQCEASAVPSAEFQWYKDDKRLIEGKKGVKVENRPFLSKLIFFNVSEHDYGNYTCVASNKLGHTNASIMLFGETVL

相同性解析による交差性が予測される生物種
Full Name neurotrimin
Calculated molecular weight 38 kDa
Observed molecular weight 58 kDa, 65 kDa
GenBank accession numberBC050716
Gene Symbol NTM
Gene ID (NCBI) 50863
RRIDAB_2878048
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P121
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neurotrimin (NTM) is a glycosylphosphatidylinositol (GPI)-anchored neural cell adhesion molecule. Neurotrimin together with the limbic system-associated protein (LAMP) and the opioid binding cell adhesion molecule (OBCAM) comprise the IgLON family of the immunoglobulin superfamily. Neurotrimin may be promoting neurite outgrowth and adhesion via a homophilic mechanism.

Protocols

Product Specific Protocols
WB protocol for NTM antibody 14373-1-APDownload protocol
IHC protocol for NTM antibody 14373-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols