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  • Featured Product
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NT5C2 Polyclonal antibody

NT5C2 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15223-1-AP

Synonyms

5' nucleotidase, cytosolic II, cN II, Cytosolic 5 nucleotidase II, GMP, NT5B, NT5C2, NT5CP, PNT5

Validation Data Gallery

Applications:
  • WB analysis using 15223-1-AP

    WB analysis using 15223-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15223-1-AP (NT5C2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 15223-1-AP

    WB analysis using 15223-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15223-1-AP (NT5C2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of human placenta using 15223-1-AP

    WB analysis of human placenta using 15223-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 15223-1-AP (NT5C2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis using 15223-1-AP

    WB analysis using 15223-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15223-1-AP (NT5C2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 15223-1-AP

    WB analysis of A549 using 15223-1-AP

    WB result of NT5C2 antibody (15223-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NT5C2 transfected A549 cells.

  • IF Staining of HepG2 using 15223-1-AP

    IF Staining of HepG2 using 15223-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using NT5C2 antibody (15223-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, human placenta tissue, A549 cells, NCl-H1299 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue, NCI-H1299 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

15223-1-AP targets NT5C2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NT5C2 fusion protein Ag7438 相同性解析による交差性が予測される生物種
Full Name 5'-nucleotidase, cytosolic II
Calculated molecular weight 65 kDa
Observed molecular weight 60-65 kDa
GenBank accession numberBC001595
Gene symbol NT5C2
Gene ID (NCBI) 22978
RRIDAB_2153946
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NT5C2 antibody 15223-1-APDownload protocol
IF protocol for NT5C2 antibody 15223-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

BMC Med Genomics

Identification of differential gene expression profile from peripheral blood cells of military pilots with hypertension by RNA sequencing analysis.

Authors - Xing-Cheng Zhao
View Article
mouseWB,IHC

Cell Death Dis

Inosine enhances tumor mitochondrial respiration by inducing Rag GTPases and nascent protein synthesis under nutrient starvation

Authors - Mei-Xin Li
View Article