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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NT5C Polyclonal antibody

NT5C Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 16577-1-AP

Synonyms

5', 3' nucleotidase, cytosolic, cdN, Deoxy 5 nucleotidase 1, DNT, dNT 1, DNT1, NT5C, P5N2, PN I, PN II, UMPH2

Validation Data Gallery

Applications:
  • IHC staining of mouse skeletal muscle using 16577-1-AP

    IHC staining of mouse skeletal muscle using 16577-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16577-1-AP (NT5C antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 16577-1-AP

    IHC staining of mouse skeletal muscle using 16577-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16577-1-AP (NT5C antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas using 16577-1-AP

    IHC staining of human pancreas using 16577-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16577-1-AP (NT5C Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human pancreas using 16577-1-AP

    IHC staining of human pancreas using 16577-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16577-1-AP (NT5C Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive IHC detected inmouse skeletal muscle tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16577-1-AP targets NT5C in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NT5C fusion protein Ag9862 相同性解析による交差性が予測される生物種
Full Name 5', 3'-nucleotidase, cytosolic
Calculated molecular weight 201 aa, 23 kDa
GenBank accession numberBC017454
Gene symbol NT5C
Gene ID (NCBI) 30833
RRIDAB_2153940
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NT5C, also named as DNT1 and UMPH2, has two isoforms with calculated MW 23 kDa and 13 kDa.

Protocols

Product Specific Protocols
IHC protocol for NT5C antibody 16577-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols