NSUN2 Recombinant antibody
NSUN2 Recombinant Antibody for WB, IF, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, IF, ELISA
Conjugate
Unconjugated
CloneNo.
230186G2
Cat no : 82894-2-RR
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, HT-1080 cells |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF) | IF : 1:150-1:600 |
| Sample-dependent, check data in validation data gallery | |
Product Information
82894-2-RR targets NSUN2 in WB, IF, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | NSUN2 fusion protein Ag14791 相同性解析による交差性が予測される生物種 |
| Full Name | NOL1/NOP2/Sun domain family, member 2 |
| Calculated molecular weight | 767 aa, 86 kDa |
| Observed molecular weight | 90-100 kDa |
| GenBank accession number | BC001041 |
| Gene symbol | NSUN2 |
| Gene ID (NCBI) | 54888 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NSUN2 antibody 82894-2-RR | Download protocol |
| IF protocol for NSUN2 antibody 82894-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
