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Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using NSUN2 Monoclonal antibody (66580-1-Ig)

    WB analysis of COLO 320 using 66580-1-Ig (same clone as 66580-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66580-1-Ig (NSUN2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using NSUN2 Monoclonal antibody (66580-1-Ig)

    WB analysis of HepG2 using 66580-1-Ig (same clone as 66580-1-PBS)

    WB result of NSUN2 antibody (66580-1-Ig; 1:40000; room temperature for 1.5 hours) with negative control and NSUN2 overexpressed HepG2 cells. This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using NSUN2 Monoclonal antibody (66580-1-Ig)

    IP experiment of HepG2 using 66580-1-Ig (same clone as 66580-1-PBS)

    IP result of anti-NSUN2 (IP:66580-1-Ig, 4ug; Detection:66580-1-Ig 1:4000) with HepG2 cells lysate 1920 ug. This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using NSUN2 Monoclonal antibody (66580-1-Ig)

    IHC staining of human oesophagus cancer using 66580-1-Ig (same clone as 66580-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66580-1-Ig (NSUN2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NSUN2 Monoclonal antibody (66580-1-Ig)

    IHC staining of human breast cancer using 66580-1-Ig (same clone as 66580-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66580-1-Ig (NSUN2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NSUN2 Monoclonal antibody (66580-1-Ig)

    IHC staining of human breast cancer using 66580-1-Ig (same clone as 66580-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66580-1-Ig (NSUN2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NSUN2 Monoclonal antibody (66580-1-Ig)

    IF Staining of HepG2 using 66580-1-Ig (same clone as 66580-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66580-1-Ig (NSUN2 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NSUN2 Monoclonal antibody, PBS Only

NSUN2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66580-1-PBS
Clone No.2H5A2

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

hTrm4, MISU, NSUN2, SAKI, TRM4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66580-1-PBS targets NSUN2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag14791

Product name: Recombinant human NSUN2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 415-767 aa of BC001041

Sequence: HHQNTGGFFVAVLVKKSSMPWNKRQPKLQGKSAETRESTQLSPADLTEGKPTDPSKLESPSFTGTGDTEIAHATEDLENNGSKKDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSSETYSQAKDLAKGSIVLKYEPDSANPDALQCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLGEKKKEGVILTNESAASTGQPDNDVTEGQRAGEPNSPDAEEANSPDVTAGCDPAGVHPPR

相同性解析による交差性が予測される生物種
Full Name NOL1/NOP2/Sun domain family, member 2
Calculated molecular weight 767 aa, 86 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC001041
Gene Symbol NSUN2
Gene ID (NCBI) 54888
RRIDAB_2881940
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ08J23
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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