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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NSMAF Polyclonal antibody (19407-1-AP)

    WB analysis using 19407-1-AP (same clone as 19407-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19407-1-AP (NSMAF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19407-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using NSMAF Polyclonal antibody (19407-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 19407-1-AP (same clone as 19407-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 19407-1-AP (NSMAF antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19407-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using NSMAF Polyclonal antibody (19407-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 19407-1-AP (same clone as 19407-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 19407-1-AP (NSMAF antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19407-1-PBS in a different storage buffer formulation.

NSMAF Polyclonal antibody, PBS Only

NSMAF Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 19407-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Protein FAN, FAN, Factor associated with N-SMase activation

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19407-1-PBS targets NSMAF in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5730

Product name: Recombinant human NSMAF protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 568-917 aa of BC041124

Sequence: FVTPHPRRITPKFKSLSQTSSYNASMADSPGEESFEDLTEESKTLAWNNITKLQLHEHYKIHKEAVTGITVSRNGSSVFTTSQDSTLKMFSKESKMLQRSISFSNMALSSCLLLPGDATVITSSWDNNVYFYSIAFGRRQDTLMGHDDAVSKICWHDNRLYSASWDSTVKVWSGVPAEMPGTKRHHFDLLAELEHDVSVDTISLNAASTLLVSGTKEGTVNIWDLTTATLMHQIPCHSGIVCDTAFSPDSRHVLSTGTDGCLNVIDVQTGMLISSMTSDEPQRCFVWDGNSVLSGSQSGELLVWDLLGAKISERIQGHTGAVTCIWMNEQCSSIITGGEDRQIIFWKLQY

相同性解析による交差性が予測される生物種
Full Name neutral sphingomyelinase (N-SMase) activation associated factor
Calculated molecular weight 104 kDa
Observed molecular weight100-104 kDa
GenBank accession numberBC041124
Gene Symbol NSMAF
Gene ID (NCBI) 8439
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92636
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NSMAF is a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation.

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