Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 15620-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15620-1-AP (NSFL1C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 15620-1-AP
WB result of NSFL1C antibody (15620-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSFL1C transfected A431 cells.
WB analysis using 15620-1-AP
Mouse liver tissue was subjected to SDS-PAGE followed by western blot with 15620-1-AP (NSFL1C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15620-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15620-1-AP (NSFL1C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 15620-1-AP
IP result of anti-NSFL1C (IP:15620-1-AP, 4ug; Detection:15620-1-AP 1:1000) with HEK-293 cells lysate 2640ug.
IHC staining of human lung cancer using 15620-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15620-1-AP (NSFL1C Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 15620-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15620-1-AP (NSFL1C Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver cancer using 15620-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15620-1-AP (NSFL1C Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 15620-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15620-1-AP (NSFL1C Antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

NSFL1C Polyclonal antibody

NSFL1C Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 15620-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Synonyms

NSFL1 cofactor p47, p97 cofactor p47, UBX domain-containing protein 2C, UBXN2C

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, mouse liver tissue, A431 cells, HeLa cells, U-251 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human lung cancer tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 6 publications below
IF	See 1 publications below

Product Information

15620-1-AP targets NSFL1C in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8040 Product name: Recombinant human NSFL1C protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-370 aa of BC002801 Sequence: MAAERQEALREFVAVTGAEEDRARFFLESAGWDLQIALASFYEDGGDEDIVTISQATPSSVSRGTAPSDNRVTSFRDLIHDQDEDEEEEEGQRFYAGGSERSGQQIVGPPRKKSPNELVDDLFKGAKEHGAVAVERVTKSPGETSKPRPFAGGGYRLGAAPEEESAYVAGEKRQHSSQDVHVVLKLWKSGFSLDNGELRSYQDPSNAQFLESIRRGEVPAELRRLAHGGQVNLDMEDHRDEDFVKPKGAFKAFTGEGQKLGSTAPQVLSTSSPAQQAENEAKASSSILIDESEPTTNIQIRLADGGRLVQKFNHSHRISDIRLFIVDARPAMAATSFILMTTFPNKELADESQTLKEANLLNAVIVQRLT 相同性解析による交差性が予測される生物種
Full Name	NSFL1 (p97) cofactor (p47)
Calculated molecular weight	41 kDa
Observed molecular weight	41 kDa
GenBank accession number	BC002801
Gene Symbol	NSFL1C
Gene ID (NCBI)	55968
RRID	AB_2878158
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UNZ2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for NSFL1C antibody 15620-1-AP	Download protocol
IP protocol for NSFL1C antibody 15620-1-AP	Download protocol
WB protocol for NSFL1C antibody 15620-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A Altered cofactor regulation with disease-associated p97/VCP mutations. Authors - Xiaoyi Zhang View Article
human	WB	Cell Chem Biol Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase. Authors - Xiaojun Wu View Article
human	WB	J Biol Chem Pathogenic mutations in the Valosin-containing protein/p97(VCP) N-domain inhibit the SUMOylation of VCP and lead to impaired stress response. Authors - Tao Wang View Article
human	WB,IF	J Cell Sci The p97-UBXN1 complex regulates aggresome formation. Authors - Sirisha Mukkavalli KD Validated View Article
mouse	WB	Evid Based Complement Alternat Med Shenxian-Shengmai Oral Liquid Improves Sinoatrial Node Dysfunction through the PKC/NOX-2 Signaling Pathway. Authors - Heng Zhang View Article
human	WB	Cancer Res A Genome-Wide Synthetic Lethal Screen Identifies Spermidine Synthase as a Target to Enhance Erdafitinib Efficacy in FGFR-Mutant Bladder Cancer Authors - Yanchao Yu View Article

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Validation Data Gallery

WB analysis using 15620-1-AP

WB analysis of A431 using 15620-1-AP

WB analysis using 15620-1-AP

WB analysis of mouse liver using 15620-1-AP

IP experiment of HEK-293 using 15620-1-AP

IHC staining of human lung cancer using 15620-1-AP

IHC staining of human lung cancer using 15620-1-AP

IHC staining of human liver cancer using 15620-1-AP

IHC staining of human liver cancer using 15620-1-AP

NSFL1C Polyclonal antibody

NSFL1C Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Altered cofactor regulation with disease-associated p97/VCP mutations.

Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase.

Pathogenic mutations in the Valosin-containing protein/p97(VCP) N-domain inhibit the SUMOylation of VCP and lead to impaired stress response.

The p97-UBXN1 complex regulates aggresome formation.

Shenxian-Shengmai Oral Liquid Improves Sinoatrial Node Dysfunction through the PKC/NOX-2 Signaling Pathway.

A Genome-Wide Synthetic Lethal Screen Identifies Spermidine Synthase as a Target to Enhance Erdafitinib Efficacy in FGFR-Mutant Bladder Cancer

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Validation Data Gallery

WB analysis using 15620-1-AP

WB analysis of A431 using 15620-1-AP

WB analysis using 15620-1-AP

WB analysis of mouse liver using 15620-1-AP

IP experiment of HEK-293 using 15620-1-AP

IHC staining of human lung cancer using 15620-1-AP

IHC staining of human lung cancer using 15620-1-AP

IHC staining of human liver cancer using 15620-1-AP

IHC staining of human liver cancer using 15620-1-AP

NSFL1C Polyclonal antibody

NSFL1C Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Altered cofactor regulation with disease-associated p97/VCP mutations.

Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase.

Pathogenic mutations in the Valosin-containing protein/p97(VCP) N-domain inhibit the SUMOylation of VCP and lead to impaired stress response.

The p97-UBXN1 complex regulates aggresome formation.

Shenxian-Shengmai Oral Liquid Improves Sinoatrial Node Dysfunction through the PKC/NOX-2 Signaling Pathway.

A Genome-Wide Synthetic Lethal Screen Identifies Spermidine Synthase as a Target to Enhance Erdafitinib Efficacy in FGFR-Mutant Bladder Cancer

Able™

Able^™