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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using NSBP1 Polyclonal antibody (23955-1-AP)

    WB analysis of PC-3 using 23955-1-AP (same clone as 23955-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 23955-1-AP (NSBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23955-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NSBP1 Polyclonal antibody (23955-1-AP)

    WB analysis of HeLa using 23955-1-AP (same clone as 23955-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 23955-1-AP (NSBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23955-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using NSBP1 Polyclonal antibody (23955-1-AP)

    WB analysis of HepG2 using 23955-1-AP (same clone as 23955-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23955-1-AP (NSBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23955-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using NSBP1 Polyclonal antibody (23955-1-AP)

    IHC staining of human testis using 23955-1-AP (same clone as 23955-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 23955-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using NSBP1 Polyclonal antibody (23955-1-AP)

    IHC staining of human testis using 23955-1-AP (same clone as 23955-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 23955-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using NSBP1 Polyclonal antibody (23955-1-AP)

    IHC staining of human testis using 23955-1-AP (same clone as 23955-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 23955-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using NSBP1 Polyclonal antibody (23955-1-AP)

    IHC staining of human testis using 23955-1-AP (same clone as 23955-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 23955-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using NSBP1 Polyclonal antibody (23955-1-AP)

    IHC staining of human testis using 23955-1-AP (same clone as 23955-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 23955-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using NSBP1 Polyclonal antibody (23955-1-AP)

    IHC staining of human testis using 23955-1-AP (same clone as 23955-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 23955-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NSBP1 Polyclonal antibody (23955-1-AP)

    IF Staining of HepG2 using 23955-1-AP (same clone as 23955-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSBP1 antibody (23955-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 23955-1-PBS in a different storage buffer formulation.

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NSBP1 Polyclonal antibody, PBS Only

NSBP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 23955-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Nucleosome-binding protein 1, NSBP 1, HMGN5, High mobility group nucleosome-binding domain-containing protein 5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23955-1-PBS targets NSBP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NSBP1 fusion protein Ag20884 相同性解析による交差性が予測される生物種
Full Name nucleosomal binding protein 1
Calculated molecular weight 282 aa, 32 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC005342
Gene Symbol NSBP1
Gene ID (NCBI) 79366
RRIDAB_2879374
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP82970
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NSBP1, also named Nucleosome-binding protein 1 or HMGN5, is a 282 amino acid protein, which belongs to the HMGN family. NSBP1 localizes in the nucleus and is ubiquitously expressed. NSBP1 preferentially binds to euchromatin and modulates cellular transcription by counteracting linker histone-mediated chromatin compaction. Western blots shows the expression of NSBP1 as a 55kDa protein in human bladder cancer cells. (PMID: 21695596).