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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NRXN1 Polyclonal antibody (55051-1-AP)

    WB analysis using 55051-1-AP (same clone as 55051-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55051-1-AP (NRXN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55051-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NRXN1 Polyclonal antibody (55051-1-AP)

    IHC staining of mouse brain using 55051-1-AP (same clone as 55051-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55051-1-AP (NRXN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55051-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NRXN1 Polyclonal antibody (55051-1-AP)

    IHC staining of mouse brain using 55051-1-AP (same clone as 55051-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55051-1-AP (NRXN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55051-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using NRXN1 Polyclonal antibody (55051-1-AP)

    IF Staining of mouse brain using 55051-1-AP (same clone as 55051-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NRXN1 antibody (55051-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55051-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using NRXN1 Polyclonal antibody (55051-1-AP)

    IF Staining of mouse brain using 55051-1-AP (same clone as 55051-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NRXN1 antibody (55051-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55051-1-PBS in a different storage buffer formulation.

NRXN1 Polyclonal antibody, PBS Only

NRXN1 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 55051-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

KIAA0578, neurexin 1, Neurexin 1 alpha, Neurexin I alpha, Neurexin I-alpha

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55051-1-PBS targets NRXN1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name neurexin 1
Calculated molecular weight 162 kDa
Observed molecular weight162 kDa
GenBank accession numberNM_004801
Gene Symbol NRXN1
Gene ID (NCBI) 9378
RRIDAB_10859257
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULB1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NRXN1 (also known as NRX1A and KIAA0578) is a member of the neurexin family. It is a neuronal cell surface protein that may be involved in cell recognition and cell adhesion. NRXN1 may mediate intracellular signaling. The antibody is specific to NRXN1 alpha.

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