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Validation Data Gallery

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  • Western Blot (WB) analysis of U-87 MG cells using Neuropilin 2 Recombinant antibody (86126-3-RR)

    WB analysis of U-87 MG using 86126-3-RR (same clone as 86126-3-PBS)

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 86126-3-RR (NRP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86126-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP02281-2

    Sandwich ELISA standard curve of MP02281-2

    Sandwich ELISA standard curve of MP02281-2, Human Neuropilin 2 Recombinant Matched Antibody Pair - PBS only. 86126-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3125. 86126-3-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

  • Sample test of MP02281-2

    Sample test of MP02281-2

    Serum of six individual healthy human donors was measured. The human Neuropilin 2 concentration of detected samples was determined to be 28.25 ng/mL with a range of 13.50 - 41.26 ng/mL.

  • Immunofluorescence (IF) / fluorescent staining of U-87 MG cells using Neuropilin 2 Recombinant antibody (86126-3-RR)

    IF Staining of U-87 MG using 86126-3-RR (same clone as 86126-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using Neuropilin 2 antibody (86126-3-RR, Clone: 250688C6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86126-3-PBS in a different storage buffer formulation.

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Neuropilin 2 Recombinant antibody, PBS Only (Detector)

Neuropilin 2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 86126-3-PBS
Clone No.250688C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

NRP2, Neuropilin-2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86126-3-PBS targets Neuropilin 2 as part of a matched antibody pair:

MP02281-2: 86126-2-PBS capture and 86126-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Neuropilin 2 fusion protein Eg3125 相同性解析による交差性が予測される生物種
Full Name neuropilin 2
Calculated molecular weight105 kDa
Observed molecular weight130 kDa
GenBank accession numberNM_201266.1
Gene Symbol Neuropilin 2
Gene ID (NCBI) 8828
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60462-1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Neuropilin 2 (NRP2) is a single-chain transmembrane glycoprotein that acts as a co-receptor of VEGF. NRP2 is mainly expressed in lymphatic and venous endothelial cells, and it is associated with the occurrence and development of several types of cancer. NRP2 is known to play a role in cardiovascular development, axon guidance, tumorigenesis, inflammation and cardiovascular disease.