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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NRIP3 Polyclonal antibody

NRIP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15664-1-AP

Synonyms

C11orf14, NRIP3, NY SAR 105, Sarcoma antigen NY SAR 105

Validation Data Gallery

Applications:
  • WB analysis of MCF-7 using 15664-1-AP

    WB analysis of MCF-7 using 15664-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 15664-1-AP (NRIP3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 15664-1-AP

    WB analysis of HeLa using 15664-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15664-1-AP (NRIP3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse ovary using 15664-1-AP

    IHC staining of mouse ovary using 15664-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 15664-1-AP (NRIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 15664-1-AP

    IHC staining of mouse testis using 15664-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15664-1-AP (NRIP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 15664-1-AP

    IHC staining of mouse testis using 15664-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15664-1-AP (NRIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse ovary using 15664-1-AP

    IHC staining of mouse ovary using 15664-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 15664-1-AP (NRIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inmouse ovary tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15664-1-AP targets NRIP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NRIP3 fusion protein Ag8097 相同性解析による交差性が予測される生物種
Full Name nuclear receptor interacting protein 3
Calculated molecular weight 241 aa, 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC008835
Gene symbol NRIP3
Gene ID (NCBI) 56675
RRIDAB_2878165
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NRIP3 antibody 15664-1-APDownload protocol
IHC protocol for NRIP3 antibody 15664-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols