Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 16396-1-AP (same clone as 16396-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16396-1-PBS in a different storage buffer formulation.
WB analysis using 16396-1-AP (same clone as 16396-1-PBS)
Non-treated HepG2 and MG132 treated HepG2 cells were subjected to SDS PAGE followed by western blot with 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16396-1-PBS in a different storage buffer formulation.
WB analysis using 16396-1-AP (same clone as 16396-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16396-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 16396-1-AP (same clone as 16396-1-PBS)
WB result of NRF2, NFE2L2 antibody (16396-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NRF2, NFE2L2 transfected HeLa cells. This data was developed using the same antibody clone with 16396-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 16396-1-AP (same clone as 16396-1-PBS)
IP result of anti-NRF2, NFE2L2 (IP:16396-1-AP, 4ug; Detection:16396-1-AP 1:6000) with HeLa cells lysate 1520 ug. This data was developed using the same antibody clone with 16396-1-PBS in a different storage buffer formulation.
IP experiment of HepG2 using 16396-1-AP (same clone as 16396-1-PBS)
IP result of anti-NRF2, NFE2L2 (IP:16396-1-AP, 4ug; Detection:16396-1-AP 1:3000) with HepG2 cells lysate 2000 ug. This data was developed using the same antibody clone with 16396-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 16396-1-AP (same clone as 16396-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16396-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 16396-1-AP (same clone as 16396-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16396-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 16396-1-AP (same clone as 16396-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16396-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 16396-1-AP (same clone as 16396-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16396-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 16396-1-AP (same clone as 16396-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16396-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 16396-1-AP (same clone as 16396-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16396-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 16396-1-AP (same clone as 16396-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16396-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 16396-1-AP (same clone as 16396-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16396-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16396-1-AP (same clone as 16396-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16396-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16396-1-AP (same clone as 16396-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16396-1-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 16396-1-AP (same clone as 16396-1-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16396-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 16396-1-AP (same clone as 16396-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed MG132 treated HepG2 cells using NRF2, NFE2L2 antibody (16396-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16396-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

NRF2, NFE2L2 Polyclonal antibody, PBS Only

NRF2, NFE2L2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16396-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NFE2L2, NRF2, HEBP1, NF-E2-related factor 2, NFE2-related factor 2

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

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MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16396-1-PBS targets NRF2, NFE2L2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9489 Product name: Recombinant human NRF2, NFE2L2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 256-605 aa of BC011558 Sequence: SILSTEDPNQLTVNSLNSDATVNTDFGDEFYSAFIAEPSISNSMPSPATLSHSLSELLNGPIDVSDLSLCKAFNQNHPESTAEFNDSDSGISLNTSPSVASPEHSVESSSYGDTLLGLSDSEVEELDSAPGSVKQNGPKTPVHSSGDMVQPLSPSQGQSTHVHDAQCENTPEKELPVSPGHRKTPFTKDKHSSRLEAHLTRDELRAKALHIPFPVEKIINLPVVDFNEMMSKEQFNEAQLALIRDIRRRGKNKVAAQNCRKRKLENIVELEQDLDHLKDEKEKLLKEKGENDKSLHLLKKQLSTLYLEVFSMLRDEDGKPYSPSEYSLQQTRDGNVFLVPKSKKPDVKKN 相同性解析による交差性が予測される生物種
Full Name	nuclear factor (erythroid-derived 2)-like 2
Calculated molecular weight	605 aa, 68 kDa
Observed molecular weight	110 kDa, 68 kDa
GenBank accession number	BC011558
Gene Symbol	NRF2
Gene ID (NCBI)	4780
RRID	AB_2782956
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16236
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16396-1-AP (same clone as 16396-1-PBS)

WB analysis using 16396-1-AP (same clone as 16396-1-PBS)

WB analysis using 16396-1-AP (same clone as 16396-1-PBS)

WB analysis of HeLa using 16396-1-AP (same clone as 16396-1-PBS)

IP experiment of HeLa using 16396-1-AP (same clone as 16396-1-PBS)

IP experiment of HepG2 using 16396-1-AP (same clone as 16396-1-PBS)

IHC staining of human pancreas cancer using 16396-1-AP (same clone as 16396-1-PBS)

IHC staining of human pancreas cancer using 16396-1-AP (same clone as 16396-1-PBS)

IHC staining of human colon cancer using 16396-1-AP (same clone as 16396-1-PBS)

IHC staining of human colon cancer using 16396-1-AP (same clone as 16396-1-PBS)

IHC staining of human liver cancer using 16396-1-AP (same clone as 16396-1-PBS)

IHC staining of human liver cancer using 16396-1-AP (same clone as 16396-1-PBS)

IHC staining of human breast cancer using 16396-1-AP (same clone as 16396-1-PBS)

IHC staining of human breast cancer using 16396-1-AP (same clone as 16396-1-PBS)

IHC staining of human kidney using 16396-1-AP (same clone as 16396-1-PBS)

IHC staining of human kidney using 16396-1-AP (same clone as 16396-1-PBS)

IHC staining of human renal cell carcinoma using 16396-1-AP (same clone as 16396-1-PBS)

IF Staining of HepG2 using 16396-1-AP (same clone as 16396-1-PBS)

NRF2, NFE2L2 Polyclonal antibody, PBS Only

NRF2, NFE2L2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information