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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NRD1 Polyclonal antibody (15630-1-AP)

    WB analysis using 15630-1-AP (same clone as 15630-1-PBS)

    K-562 cells lysates were subjected to SDS PAGE followed by western blot with 15630-1-AP (NRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15630-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NRD1 Polyclonal antibody (15630-1-AP)

    IHC staining of mouse testis using 15630-1-AP (same clone as 15630-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15630-1-AP (NRD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15630-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using NRD1 Polyclonal antibody (15630-1-AP)

    IHC staining of human testis using 15630-1-AP (same clone as 15630-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 15630-1-AP (NRD1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15630-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15630-1-PBS targets NRD1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NRD1 fusion protein Ag7923 相同性解析による交差性が予測される生物種
Full Name nardilysin (N-arginine dibasic convertase)
Calculated molecular weight 132 kDa
Observed molecular weight 135-140 kDa
GenBank accession numberBC008775
Gene Symbol NRD1
Gene ID (NCBI) 4898
RRIDAB_2154525
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43847
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NRD1(Nardilysin) is also named as NRD-C(N-arginine dibasic convertase). It is a zinc metalloendopeptidase of the M16 pitrilysin family that cleaves peptides at dibasic residues. The enzyme has also been found in neural tissues of mouse embryos indicating a role in neural development. Although nardilysin is primarily found in the cytosol, it has been reported that nardilysin is located on the cell surface acting as a receptor for heparinbinding EGF-like growth factor(PMID:15809022). It has 2 isoforms produced by alternative splicing.