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Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using NRCAM Recombinant antibody (84690-1-RR)

    WB analysis of SH-SY5Y using 84690-1-RR

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 84690-1-RR (NRCAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NRCAM Recombinant antibody (84690-1-RR)

    WB analysis using 84690-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84690-1-RR (NRCAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84690-1-RR

    Affinity and Kinetic Characterization of 84690-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84690-1-RR against Human NRCAM were performed. The affinity constant is below 1 pM.

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NRCAM Recombinant antibody

NRCAM Recombinant Antibody for WB, ELISA
Cat No. 84690-1-RR
Clone No.241862G1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Ng CAM related, Neuronal surface protein Bravo, neuronal cell adhesion molecule, KIAA0343, hBravo

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inSH-SY5Y cells, mouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84690-1-RR targets NRCAM in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NRCAM fusion protein Ag16237 相同性解析による交差性が予測される生物種
Full Name neuronal cell adhesion molecule
Calculated molecular weight 1304 aa, 144 kDa
Observed molecular weight144 kDa
GenBank accession numberBC098401
Gene Symbol NRCAM
Gene ID (NCBI) 4897
RRIDAB_3672106
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ92823
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neuronal cell adhesion molecule (NRCAM) is a member of the L1 subfamily of cell adhesion molecules (CAMs) that belong to the immunoglobulin superfamily (PMID: 11329126). NRCAM is a transmembrane protein composed of six Ig-like domains and five fibronectin type-III repeats in the extracellular region, with a highly conserved cytoplasmic tail. It is mainly expressed in the nervous system and is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. NRCAM is also expressed outside the nervous system. Altered expression of NRCAM has been associated with tumor progression in diverse organs (PMID: 22182708).

Protocols

Product Specific Protocols
WB protocol for NRCAM antibody 84690-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols