Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 10724-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10724-1-AP (NRAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of C6 using 10724-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 10724-1-AP (NRAS Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 10724-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10724-1-AP (NRAS Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10724-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10724-1-AP (NRAS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 10724-1-AP
IP result of anti-NRAS (IP:10724-1-AP, 4ug; Detection:10724-1-AP 1:4000) with HEK-293 cells lysate 1720 ug.
IHC staining of human pancreas using 10724-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10724-1-AP (NRAS Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human pancreas using 10724-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10724-1-AP (NRAS Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human testis using 10724-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10724-1-AP (NRAS antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 10724-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10724-1-AP (NRAS antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas using 10724-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10724-1-AP (NRAS Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human pancreas using 10724-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10724-1-AP (NRAS Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HeLa using 10724-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NRAS antibody (10724-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).
FC experiment of HepG2 using 10724-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NRAS Polyclonal antibody (10724-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

NRAS Polyclonal antibody

NRAS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10724-1-AP

Publications(34)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

HRAS1, EC:3.6.5.2, GTPase NRas, N ras, NRAS1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 555 CoraLite® Plus 647

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (34)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, C6 cells, NIH/3T3 cells, Jurkat cells, A549 cells, HEK-293 cells, MCF-7 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human pancreas tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 29 publications below
IHC	See 5 publications below
IF	See 4 publications below
IP	See 1 publications below

Product Information

10724-1-AP targets NRAS in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1081 Product name: Recombinant human NRAS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-189 aa of BC005219 Sequence: MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNSKSFADINLYREQIKRVKDSDDVPMVLVGNKCDLPTRTVDTKQAHELAKSYGIPFIETSAKTRQGVEDAFYTLVREIRQYRMKKLNSSDDGTQGCMGLPCVVM 相同性解析による交差性が予測される生物種
Full Name	neuroblastoma RAS viral (v-ras) oncogene homolog
Calculated molecular weight	21 kDa
Observed molecular weight	21 kDa
GenBank accession number	BC005219
Gene Symbol	NRAS
Gene ID (NCBI)	4893
RRID	AB_2154209
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P01111
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NRAS, also named as N-ras and NRAS1, is neuroblastoma RAS viral (v-ras) oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML).

Protocols

Product Specific Protocols
IF protocol for NRAS antibody 10724-1-AP	Download protocol
IHC protocol for NRAS antibody 10724-1-AP	Download protocol
IP protocol for NRAS antibody 10724-1-AP	Download protocol
WB protocol for NRAS antibody 10724-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nat Cell Biol ADAR1 downregulation by autophagy drives senescence independently of RNA editing by enhancing p16INK4a levels. Authors - Xue Hao View Article
human	WB	Gut RNA helicase DDX5 enables STAT1 mRNA translation and interferon signalling in hepatitis B virus replicating hepatocytes. Authors - Jiazeng Sun View Article
human	WB	Nat Commun Targeting NRAS via miR-1304-5p or farnesyltransferase inhibition confers sensitivity to ALK inhibitors in ALK-mutant neuroblastoma Authors - Perla Pucci View Article
human	IF	Nat Commun KRAS regulation by small non-coding RNAs and SNARE proteins. Authors - Yonglu Che View Article
mouse,human	WB	Biomaterials Polymeric nanoparticle mediated inhibition of miR-21 with enhanced miR-124 expression for combinatorial glioblastoma therapy. Authors - Yuanyuan Liu View Article
human	WB	Clin Cancer Res Combinations with Allosteric SHP2 Inhibitor TNO155 to Block Receptor Tyrosine Kinase Signaling. Authors - Chen Liu KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10724-1-AP

WB analysis of C6 using 10724-1-AP

WB analysis of NIH/3T3 using 10724-1-AP

WB analysis of Jurkat using 10724-1-AP

IP experiment of HEK-293 using 10724-1-AP

IHC staining of human pancreas using 10724-1-AP

IHC staining of human pancreas using 10724-1-AP

IHC staining of human testis using 10724-1-AP

IHC staining of human testis using 10724-1-AP

IHC staining of human pancreas using 10724-1-AP

IHC staining of human pancreas using 10724-1-AP

IF Staining of HeLa using 10724-1-AP

FC experiment of HepG2 using 10724-1-AP

NRAS Polyclonal antibody

NRAS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

ADAR1 downregulation by autophagy drives senescence independently of RNA editing by enhancing p16INK4a levels.

RNA helicase DDX5 enables STAT1 mRNA translation and interferon signalling in hepatitis B virus replicating hepatocytes.

Targeting NRAS via miR-1304-5p or farnesyltransferase inhibition confers sensitivity to ALK inhibitors in ALK-mutant neuroblastoma

KRAS regulation by small non-coding RNAs and SNARE proteins.

Polymeric nanoparticle mediated inhibition of miR-21 with enhanced miR-124 expression for combinatorial glioblastoma therapy.

Combinations with Allosteric SHP2 Inhibitor TNO155 to Block Receptor Tyrosine Kinase Signaling.