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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NRAMP1 Polyclonal antibody (32308-1-AP)

    WB analysis using 32308-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32308-1-AP (NRAMP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NRAMP1 Polyclonal antibody (32308-1-AP)

    WB analysis using 32308-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32308-1-AP (NRAMP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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NRAMP1 Polyclonal antibody

NRAMP1 Polyclonal Antibody for WB, ELISA
Cat No. 32308-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

SLC11A1, NRAMP 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHL-60 cells, Jurkat cells, THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32308-1-AP targets NRAMP1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1
Calculated molecular weight 432 aa, 48 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC071165
Gene Symbol SLC11A1
Gene ID (NCBI) 6556
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP49279
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC11A1 also known as NRAMP1, belongs to the SLC11 family of solute carriers, which mediate the transport of divalent metals. NRAMP1 is a divalent metal importer specifically expressed in the membranes of late endosomes/lysosomes of professional phagocytes. It plays a crucial role in the defense against intracellular pathogens by depleting essential metal ions from endocytosed microbes, thereby preventing their proliferation(PMID: 24832660, PMID: 39824808).

Protocols

Product Specific Protocols
WB protocol for NRAMP1 antibody 32308-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols