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- KD/KO Validated
NR4A1 Polyclonal antibody
NR4A1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 25851-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | HL-60 cells, PC-3 cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy NR4A1 Ready-To-Use IHC Kit. NR4A1 primary antibody included.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 11 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
Product Information
25851-1-AP targets NR4A1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NR4A1 fusion protein Ag23088 相同性解析による交差性が予測される生物種 |
| Full Name | nuclear receptor subfamily 4, group A, member 1 |
| Calculated molecular weight | 598 aa, 64 kDa |
| Observed molecular weight | 64-68 kDa |
| GenBank accession number | BC016147 |
| Gene symbol | NR4A1 |
| Gene ID (NCBI) | 3164 |
| RRID | AB_2880269 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NR4A1 antibody 25851-1-AP | Download protocol |
| IHC protocol for NR4A1 antibody 25851-1-AP | Download protocol |
| IP protocol for NR4A1 antibody 25851-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Mol Med NR4A1 retards adipocyte differentiation or maturation via enhancing GATA2 and p53 expression.
| ||
Phytomedicine Stigmasterol upregulates immediate early genes and promotes neuronal cytoarchitecture in primary hippocampal neurons as revealed by transcriptome analysis. | ||
Front Mol Biosci Single-Cell Profiles of Age-Related Osteoarthritis Uncover Underlying Heterogeneity Associated With Disease Progression.
| ||
Am J Physiol Endocrinol Metab Frequent and mild scrotal heat stress in mice epigenetically alters glucose metabolism in the male offspring. | ||
Front Physiol Sleeve Gastrectomy-Induced AMPK Activation Attenuates Diabetic Cardiomyopathy by Maintaining Mitochondrial Homeostasis via NR4A1 Suppression in Rats. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Angela (Verified Customer) (06-23-2020) | 35ug total protein/lane1- Untransfected HEK2932- Transfection with human NR4A1 expression vector3- Transfection with rat NR4A1 expression vector
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