Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of mouse kidney using 21854-1-AP (same clone as 21854-1-PBS)
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21854-1-AP (NR3C2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 21854-1-AP (same clone as 21854-1-PBS)
WB result of NR3C2 antibody (21854-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NR3C2 transfected HEK-293 cells. This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.
WB analysis using 21854-1-AP (same clone as 21854-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 21854-1-AP (NR3C2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.
IP experiment of HEK-293 using 21854-1-AP (same clone as 21854-1-PBS)
IP result of anti-NR3C2 (IP:21854-1-AP, 4ug; Detection:21854-1-AP 1:500) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 21854-1-AP (same clone as 21854-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 21854-1-AP (same clone as 21854-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 21854-1-AP (same clone as 21854-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 21854-1-AP (same clone as 21854-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 21854-1-AP (same clone as 21854-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 21854-1-AP (same clone as 21854-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 21854-1-AP (same clone as 21854-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NR3C2 antibody (21854-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.
IF Staining of HEK-293 using 21854-1-AP (same clone as 21854-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using NR3C2 antibody (21854-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

NR3C2 Polyclonal antibody, PBS Only

NR3C2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 21854-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

MCR, MLR, MR

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21854-1-PBS targets NR3C2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16410 Product name: Recombinant human NR3C2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC111758 Sequence: METKGYHSLPEGLDMERRWGQVSQAVERSSLGPTERTDENNYMEIVNVSCVSGAIPNNSTQGSSKEKQELLPCLQQDNNRPGILTSDIKTELESKELSATVAESMGLYMDSVRDADYSYEQQNQQGSMSPAKIYQNVEQLVKFYKGNGHRPSTLSCVNTPLRSFMSDSGSSVNGGVMRAIVKSPIMCHEKSPSVCSPLNMTSSVCSPAGINSVSSTTASFGSFPVHSPITQGTPLTCSPNVENRGSRSHSPAHASNVGSPLSSPLSSMKSSISSPPSHCSVKSPVSSPNNVTLRSSVSSPANINNSRCSVSSPSNTNNRSTLSSPAASTVGSICSPVNNAFSYTASGTSA 相同性解析による交差性が予測される生物種
Full Name	nuclear receptor subfamily 3, group C, member 2
Calculated molecular weight	984 aa, 107 kDa
Observed molecular weight	94-110 kDa
GenBank accession number	BC111758
Gene Symbol	NR3C2
Gene ID (NCBI)	4306
RRID	AB_10858785
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P08235
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Nuclear receptor subfamily 3 group C member 2 (NR3C2), also known as Mineralocorticoid receptor (MCR or MR), is a member of the steroid/thyroid /retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor down-regulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation). MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse kidney using 21854-1-AP (same clone as 21854-1-PBS)

WB analysis of HEK-293 using 21854-1-AP (same clone as 21854-1-PBS)

WB analysis using 21854-1-AP (same clone as 21854-1-PBS)

IP experiment of HEK-293 using 21854-1-AP (same clone as 21854-1-PBS)

IHC staining of human kidney using 21854-1-AP (same clone as 21854-1-PBS)

IHC staining of human kidney using 21854-1-AP (same clone as 21854-1-PBS)

IHC staining of human colon using 21854-1-AP (same clone as 21854-1-PBS)

IHC staining of human colon using 21854-1-AP (same clone as 21854-1-PBS)

IHC staining of mouse kidney using 21854-1-AP (same clone as 21854-1-PBS)

IHC staining of mouse kidney using 21854-1-AP (same clone as 21854-1-PBS)

IF Staining of HepG2 using 21854-1-AP (same clone as 21854-1-PBS)

IF Staining of HEK-293 using 21854-1-AP (same clone as 21854-1-PBS)